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Merck
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Key Documents

MABF125

Sigma-Aldrich

Anti-TAP1 Antibody, clone mAb 148.3

clone mAb 148.3, from mouse

同義詞:

Antigen peptide transporter 1, APT1, ATP-binding cassette sub-family B member 2, Peptide supply factor 1, Peptide transporter PSF1, PSF-1, Peptide transporter TAP1, Peptide transporter involved in antigen processing 1, Really interesting new gene 4 prote

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

mAb 148.3, monoclonal

物種活性

human

技術

activity assay: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG1κ

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... TAP1(6890)

一般說明

Peptide transporter TAP1 is also called Antigen peptide transporter 1 (APT1), ATP-binding cassette sub-family B member 2 (ABCB2), Peptide supply factor 1 (PSF1), Peptide transporter PSF1 (PSF-1), Peptide transporter involved in antigen processing 1, and Really interesting new gene 4 protein (RING4). TAP1 is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules as well as MHC class I folding. TAP1 is inhibited herpes simplex virus ICP47 protein, human cytomegalovirus US6 glycoprotein, human adenovirus E3-19K glycoprotein, and is down-regulated by human Epstein-Barr virus vIL-10 protein. TAP1 mutations can be a cause of Bare lymphocyte syndrome 1 (BLS1).

免疫原

Epitope: This antibody recognizes the ADAPE amino acid residues within the CYWAMVQAPADAPE sequence of human TAP1 (located at the C-terminus).
Linear peptide corresponding to the CYWAMVQAPADAPE sequence of human TAP1.

應用

Detect Antigen peptide transporter 1 using this mouse monoclonal antibody, Anti-TAP1 Antibody, clone mAb 148.3 validated for use in western blotting, IP, Immunofluorescence & Activity Assay.
Western Blotting Analysis: A representative lot from an independent laboratory detected TAP1 in transiently transfected HeLa cells (Hulpke, S., et al. (2012). FASEB J. 26(12):5071-5080.).

Western Blotting Analysis: A representative lot from an independent laboratory detected TAP1 in microsomes of baculovirus-infected SF9 cells, which express wild type or select mutations of TAP1 (Chen, M., et al. (2004). J Biol Chem. 279(44):46073-46081.).

Western Blotting Analysis: A representative lot from an independent laboratory detected TAP1 in SF9 cells infected with recombinant baculovirus containing TAP1 gene constructs (Meyer, T. H., et al. (1994). FEBS Lett. 351(3):443-447.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected TAP1 in HeLa cells contransfected with wild type TAP1 and TAP2 (Hulpke, S., et al. (2012). Cell Mol Life Sci. 69(19):3317-3327.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected TAP1 in SF9 cells infected with rBV-TAP1/rBV-TAP2 (Meyer, T. H., et al. (1994). FEBS Lett. 351(3):443-447.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated TAP1 from SF9 cells infected with rBV-TAP1/rBV-TAP2 (Meyer, T. H., et al. (1994). FEBS Lett. 351(3):443-447.).

Activity Assay Analysis: This antibody inhibits TAP-specific peptide transport (Plewnia, G., et al. (2007). J Mol Biol. 369(1):95-107.).

品質

Evaluated by Western Blotting in untreated and Interferon-gamma (IFN-g) treated HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TAP1 in 10 µg of Interferon-gamma (IFN-g) treated HeLa cell lysate.

標靶描述

~65 kDa observed. Uniprot describes a molecular weight of ~87 kDa However, this protein may be observed at ~65 kDa (Koch, J., et al. (2006). FEBS Lett. 580(17):4091-4096.).

外觀

Format: Purified
Purified mouse monoclonal IgG1κ supernatant in buffer containing PBS without preservatives.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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存取文件庫

Min Chen et al.
The Journal of biological chemistry, 279(44), 46073-46081 (2004-08-24)
The transporter associated with antigen processing (TAP) is a key component of the cellular immune system. As a member of the ATP-binding cassette (ABC) superfamily, TAP hydrolyzes ATP to energize the transport of peptides from the cytosol into the lumen
Functional expression and purification of the ABC transporter complex associated with antigen processing (TAP) in insect cells.
Meyer, T H, et al.
Febs Letters, 351, 443-447 (1994)
Gabriele Plewnia et al.
Journal of molecular biology, 369(1), 95-107 (2007-04-10)
The transporter associated with antigen processing (TAP) plays a pivotal role in the major histocompatibility complex (MHC) class I mediated immune response against infected or malignantly transformed cells. It belongs to the ATP-binding cassette (ABC) superfamily and consists of TAP1
Man Huang et al.
Scientific reports, 6, 33612-33612 (2016-09-17)
HLA class I (HLA-I) transgenic mice have proven to be useful models for studying human MHC-related immune responses over the last two decades. However, differences in the processing and presentation machinery between humans and mice may have profound effects on
Sabine Hulpke et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(12), 5071-5080 (2012-08-28)
The loading of antigen-derived peptides onto MHC class I molecules for presentation to cytotoxic T cells is a key process in adaptive immune defense. Loading of MHC I is achieved by a sophisticated machinery, the peptide-loading complex (PLC), which is

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