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重要文件

MABE343-AF647

Sigma-Aldrich

抗-嘌呤霉素抗体,克隆12D10,Alexa Fluor 647偶联物

clone 12D10, 0.5 mg/mL, from mouse

同義詞:

Alexa Fluor 647 Anti-Puromycin, Clone 12D10 Anti-Puromycin, Puromycin Detection Antibody

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

共軛

ALEXA FLUOR 647

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

12D10, monoclonal

物種活性

human

濃度

0.5 mg/mL

技術

immunocytochemistry: suitable

運輸包裝

wet ice

目標翻譯後修改

unmodified

一般說明

嘌呤霉素是一种氨基核苷酸抗生素,来源于白黑链霉菌,是蛋白合成抑制剂,可通过核糖体中的过早链终止来阻止翻译。抗嘌呤霉素的单克隆抗体可与标准免疫化学方法一起用于直接监测翻译,这种方法称为翻译表面感应(SUnSET)。该分子的一部分类似于氨基酰化tRNA的3′端,这使其可用于蛋白质翻译分析。嘌呤霉素诱导胸腺细胞和HL-60白血病细胞内DNA断裂。
该抗嘌呤霉素(克隆12D10)抗体经过免疫细胞化学应用验证,可用于检测嘌呤霉素。

特異性

与嘌呤霉素预孵育时,预期与所有物种细胞发生反应。

品質

通过免疫细胞化学分析未处理和嘌呤霉素处理的HeLa细胞进行评估。

免疫细胞化学分析:用该抗体的1:2,500稀释液在嘌呤霉素处理的HeLa细胞中检测掺入嘌呤霉素的新合成蛋白。

Alexa Fluor是Life Technologies的注册商标。

標靶描述

请参阅货号MABE343了解观测到的分子量信息。

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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