MABE341-AF488

Anti-Puromycin, clone 17H1, Alexa Fluor^™ 488 Conjugate Antibody

clone 17H1, 0.5 mg/mL, from rat

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rat
• 品質等級: 100
• 共軛: ALEXA FLUOR^™ 488
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 17H1, monoclonal
• 物種活性: human
• 濃度: 0.5 mg/mL
• 技術: immunocytochemistry: suitable
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified

描述

一般說明

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This product is a conjugated version of Cat. No. MABE341.

特異性

Demonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.

This antibody detects Puromycin-incorporated neosynthesized proteins.

免疫原

Puromycin-incorporated neosynthesized proteins at multiple molecular weights

應用

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

This Anti-Puromycin, clone 17H1 is validated for use in Immunocytochemistry for the detection of Puromycin.

品質

Evaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.

Alexa Fluor^™ is a registered trademark of Life Technologies.

標靶描述

Refer to Cat. No. MABE341 for observed molecular weight information.

外觀

Protein G Purified

Purified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable