MABE286

Anti-Replication Protein A Antibody, clone RPA34-19

clone RPA34-19, from mouse

• 同義詞: Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RP-A p34
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: RPA34-19, monoclonal
• 物種活性: human
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_002937
• UniProt登錄號: P15927
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... RPA2(6118)

描述

一般說明

RPA (RP-A p32) is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits: RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa). RPA plays multiple roles in DNA replication. At the onset of DNA replication, RPA is loaded onto chromatin, and is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination. The 32 kDa subunit is phosphorylated by the cdc2 family of kinases when cells enter S-phase; and by ATM, ATR, and DNA-PK proteins in response to DNA damage.

免疫原

Replication Protein A purified from U293 cells.

應用

Anti-Replication Protein A Antibody, clone RPA34-19 is a Mouse Monoclonal Antibody for detection of Replication Protein A also known as Replication protein A 32 kDa subunit, RP-A p32 & has been validated in WB, ICC & IHC.

Immunocytochemistry Analysis: A 1:250 dilution from a reprsentative lot detected Replication Protein A in A431 cells.

Immunohistochemistry Analysis: A 1:5 dilution from a representative lot detected Replication Protein A in human placental chorionic villi and in human colorectal adenocarcinoma tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

品質

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected Replication Protein A in 10 µg of HeLa cell lysate.

標靶描述

~34 kDa observed. This protein has 3 isoforms: Isoform 1 (~29 kDa), Isoform 2 (~30 kDa), and Isoform 3 (~39 kDa).

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
HeLa cell lysate

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable