1.16882

Fractogel EMD SO₃⁻ (M)

• 同義詞: Fractogel^® EMD SO₃ ⁻ (M) 树脂
• 分類程式碼代碼: 41115711

屬性

• 品質等級: 400
• ligand: (Sulfoisobutyl)
• 描述: strong cation exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
• 無菌: sterile (Caustic Stable)
• 產品線: Fractogel^®
• 形狀: resin
• 特定分析物: MAbs
• 參數: 8 bar max. pressure
• 基質活性組: methacrylate
• 平均粒徑: 40-90 μm
• 容量: 130 mg binding capacity (lysozyme/ml of resin)
• 轉變溫度: flash point 35 °C (Does not sustain combustion.)
• 密度: 1.43 g/cm³ at 20 °C
• 體積密度: 1000 kg/m³
• 應用: recombinant protein; vaccine development
• 分離技術: cation exchange; strong cation exchange
• 儲存溫度: 2-30°C

描述

一般說明

Strong cation exchange Fractogel^® resin featuring the tentacle technology, for purification of basic and neutral proteins (e.g. antibodies) and peptides

特點和優勢

Fractogel^® EMD SO₃^- (M) enables:

• Increased binding strength at low pH
• High aggregate removal efficiency combined with high dynamic binding capacity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel^® EMD SO₃^- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

包裝

• 1.16882.0100: Fractogel^® EMD SO3- (M) Resin 100ml
• 1.16882.0010: Fractogel^® EMD SO3- (M) Resin 10ml
• 1.16880.0500: Fractogel^® EMD SO3- (M) Resin 500ml
• 1.16882.5000: Fractogel^® EMD SO3- (M) Resin 5L

Plastic bottle

分析報告

Appearance: Milky turbid suspension,free from impurities(foreign particles)
Microscopic evaluation: Uniform spherical particles,no agglomerates, no fines
Extractable matter (water): ≤ 0.05 %
Cerium: ≤ 1 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (lysozyme): 110 - 150 mg/ml
Functional test (b:a): ≤ 0.4
Functional test (c:d): ≤ 0.45
Functional test: Separation chymotrypsinogen A, cytochrom C and lysozyme

法律資訊

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P210 - P233 - P240 - P241 - P242 - P243
• 危險分類: Flam. Liq. 3
• 儲存類別代碼: 3 - Flammable liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: 95.0 °F
• 閃點（°C）: 35 °C