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Key Documents

MABE1132

Sigma-Aldrich

Anti-TET2 Antibody, clone 9F7

clone 9F7, from rat

同義詞:

Methylcytosine dioxygenase TET2, Protein Ayu17-449

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rat

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

9F7, monoclonal

物種活性

mouse

技術

ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG2aκ

NCBI登錄號

UniProt登錄號

目標翻譯後修改

unmodified

基因資訊

mouse ... Tet2(214133)

一般說明

Methylcytosine dioxygenase TET2 (EC 1.14.11.n2; UniProt Q4JK59; also known as Protein Ayu17-449) is encoded by the Tet2 (also known as Kiaa1546) gene (Gene ID 214133) in murine species. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine, providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported.

特異性

Clone 9F7 detected exogenously expressed TET2, but not TET1 or TET3, GST fusion (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812). Epitope region is present in all three murine TET2 spliced isoforms reported by UniProt (Q4JK59).

免疫原

Epitope: Region between DNA-binding and 2-oxoglutarate-binding domains.
His-tagged recombinant fragment from the region between DNA-binding and 2-oxoglutarate-binding domains of mouse TET2.

應用

Anti-TET2 Antibody, clone 9F7 is an antibody against TET2 for use in Immunocytochemistry, Western Blotting, Immunoprecipitation, ELISA.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Western Blotting Analysis: A representative lot detected exogenously expressed TET2, but not TET1 or TET3, GST fusion in lysates from respective HEK293T transfectants (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous TET2 from murine embryonic stem cell (mESC) lysate. Subsequent LC-MS/MS analysis detected the presence of co-immunoprecipitated glycosyltransferase OGT (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
ELISA Analysis: Clone 9F7 hybridoma culture supernatant was confirmed for its immunoreactivity against His-tagged murine TET2 by ELISA (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).

品質

Evaluated by Immunocytochemistry in mouse mouse ESCs.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected TET2 in mouse embryonic stem cells (ESCs).

標靶描述

212.1 kDa (isoform 1), 68.06 kDa (isoform 2), 64.50 kDa (isoform 3) calculated.

外觀

Protein G purified.
Format: Purified
Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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