MABD51
抗-Brn-2 (POU3F2)抗体,克隆8C4.2
clone 8C4.2, from mouse
同義詞:
POU domain, class 3, transcription factor 2, Brain-specific homeobox/POU domain protein 2, Brain-2, Brn-2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Oct-7, Octamer-binding transcription factor 7, OTF
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About This Item
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生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
8C4.2, monoclonal
物種活性
rat, human
技術
immunohistochemistry: suitable (paraffin)
western blot: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... POU3F2(5454)
一般說明
Brn-2 (POU结构域,第3类,转录因子2; Oct-7; N-Oct-3; 或POU3F2)是POU 结构域转录因子大家族的成员。 高度同源的POU结构域包含N末端的一个POU特定结构域,C末端的一个POU同源结构域,以及一个互联连接子区。POU 结构域连接 DNA 序列 5’-ATGCAAAT-3’。Brn-2 在发育中的CNS中高度表达,并可能在神经形成,特别是下丘脑的发育中起一定作用。过去的研究也表明 Brn-2 可以整合 BRAF/MAP 激酶和Wnt/β-联蛋途径下游的信号,Brn-2 可能涉及黑色素瘤的转化和增殖。
免疫原
GST标记的重组蛋白,对应于人Brn-2 (POU3F2)。
應用
免疫组化分析:代表性批次的1:50稀释液可在正常大鼠脑组织、浦肯野细胞、篮细胞、大鼠小脑组织的神经胶质细胞、大鼠小脑组织的神经元、大鼠额叶皮层的神经元和神经胶质细胞以及人脑桥组织的神经元中检测Brn-2。
抗-Brn-2(POU3F2)抗体(克隆8C4.2)可检测Brn-2 (POU3F2)水平,&已发布&经验证可用于蛋白质印迹、IHC(P)。
品質
通过蛋白质印迹法在 SK-N-MC 细胞裂解液中进行了评估。
蛋白质印迹分析:该抗体的1:2,000稀释液在10 µg SK-N-MC细胞裂解液中检测到Brn-2 (POU3F2)。
蛋白质印迹分析:该抗体的1:2,000稀释液在10 µg SK-N-MC细胞裂解液中检测到Brn-2 (POU3F2)。
標靶描述
观测值〜54 kDa。
计算分子量为47 kDa,Brn-2(POU3F2)在一些细胞裂解液中观测值为~50-55 kDa(Wolfe, A., et al., (2002).Molecular Endocrinology.16(3):435-449)。
计算分子量为47 kDa,Brn-2(POU3F2)在一些细胞裂解液中观测值为~50-55 kDa(Wolfe, A., et al., (2002).Molecular Endocrinology.16(3):435-449)。
外觀
形式:纯化
分析報告
对照
SK-N-MC细胞裂解液
SK-N-MC细胞裂解液
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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