MABD136

Anti-Wnt-5a Antibody, clone 6F2

ascites fluid, clone 6F2, from mouse

• 同義詞: Protein Wnt-5a
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: ascites fluid
• 抗體產品種類: primary antibodies
• 無性繁殖: 6F2, monoclonal
• 物種活性: human, rat
• 技術: immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG1
• UniProt登錄號: P41221
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... WNT5A(7474)

描述

一般說明

The Wnt family of cysteine-rich proteins constitutes a group of ligands known for their roles in development such as cell differentiation, proliferation, migration and embryonic patterning. They have also been implicated in the formation of tumors. Wnts are involved in the stimulation of several signaling cascades that have been divided into two types; the canonical pathway, and the non-canonical pathway. Wnt-5a is considered to be a non-canonical ligand for the Wnt signaling pathway and is a ligand to the frizzled family of seven transmembrane receptors. When interacting with ROR2, Wnt-5a is considered to inhibit tumor formation due to Wnt-5a’s suppression of the canonical Wnt signaling pathway. Wnt-5a is involved in activating cell migration, regulating the motility of melanoma cells, decreasing the proliferation, chondrogenesis, and migration of carcinoma cells, and inhibiting the expression of type II collagen in chondrocytes. Wnt-5a is a requirement to the endothelial differentiation and vascularization of Embryonic Stem Cells, and is also necessary to the development of mammary glands, limb outgrowth, and the genital tubercle.

免疫原

Purified recombinant fragment of human Wnt-5a expressed in E. Coli.

應用

Detect Wnt-5a using this mouse monoclonal antibody, Anti-Wnt-5a Antibody, clone 6F2 validated for use in western blotting, Immunofluorescence & IHC.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected Wnt-5a in human lung cancer, thyroid cancer, lymph node, and brain tissues.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected Wnt-5a in PC-12 cells.

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

品質

Evaluated by Western Blotting in Wnt-5a-hIgGFc transfected HEK293 cell lysate.

Western Blotting Analysis: A 1:500-2000 dilution of this antibody detected Wnt-5a in Wnt-5a-hIgGFc transfected HEK293 cell lysate.

標靶描述

~70 kDa observed

外觀

Unpurified

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Wnt-5a-hIgGFc transfected HEK293 cell lysate

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable