A2103

Anti-Actin, N-terminal antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 同義詞: Actin Detection Antibody, Rabbit Anti-Actin
• MDL號碼: MFCD00145889
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 42 kDa
• 物種活性: frog, rat, mouse, chicken, human
• 包裝: antibody small pack of 25 μL
• 加強驗證: independent; Learn more about Antibody Enhanced Validation
• 濃度: ~0.5 mg/mL
• 技術: immunocytochemistry: 1-2 μg/mL using cultured chicken fibroblasts; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using sections of human appendix, mouse heart, and frog skeletal muscle; indirect immunofluorescence: suitable; western blot: 0.5-1 μg/mL using whole extract of the human epitheloid carcinoma HeLa cell line.; western blot: 2-4 ng/mL using whole extract of rat skeletal muscle
• UniProt登錄號: P63261
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ACTA1(58) , ACTA2(59) , ACTB(60) , ACTC1(70) , ACTG1(71) , ACTG2(72) ; mouse ... Acta1(11459) , Acta2(11475) , Actb(11461) , Actc1(11464) , Actg1(11465) , Actg2(11468) ; rat ... Acta1(29437) , Acta2(81633) , Actb(81822) , Actc1(29275) , Actg1(287876) , Actg2(25365)

描述

一般說明

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as an antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.

Actin is one of the most conserved eukaryotic proteins, which is expressed in mammals and birds as at least six major different isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all non-muscle cells. Actin isoforms show >90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues. Actin is present in cells both as a globular monomer (G-actin) and as a polymer in filamentous actin (F-actin) that participates in the formation of a variety of stable and labile structures. The presence of actin in cell nuclei and cell membranes has been reported.

免疫原

synthetic actin N-terminal nonapeptide conjugated to KLH.

應用

Anti-Actin, N-terminal antibody produced in rabbit has been used in

• immunoblotting
• quantitative chromatin immunoprecipitation (qChIP)

Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates . Actin was detected at 42 kDa.

Rabbit anti-actin, N-terminal antibody can be used for western blotting assays. The antibody can also be used for immunocytochemistry at 1-2μg/mL using cultured chicken fibroblasts. Furthermore, the antibody is suitable for use in immunohistochemistry at a concentration of 2-4μg/mL using sections of human appendix, mouse heart, and frog skeletal muscle.

生化／生理作用

Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including maintenance of cell shape, locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The N-terminal domain of actin appears to be a major antigenic region of the molecule. Different isoforms can coexist in the same cell but are differentially regulated, and in most cases cannot substitute for each other functionally.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

其他說明

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)