MABC684

Anti-SOD1 Antibody, clone 6F5

ascites fluid, clone 6F5, from mouse

• 同義詞: ALS, SOD, ALS1, IPOA, homodimerSuperoxide dismutase [Cu-Zn], Superoxide dismutase 1, hSod1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: ascites fluid
• 抗體產品種類: primary antibodies
• 無性繁殖: 6F5, monoclonal
• 物種活性: mouse, human
• 技術: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• 同型: IgG1
• UniProt登錄號: P00441
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SOD1(6647)

描述

一般說明

Superoxide dismutase 1 (SOD1) also known as Superoxide dismutase [Cu-Zn] to distinguish it from SOD2 which uses manganese as its cofactor, is the cytosolic form of the enzyme that catalyzes the breakdown of superoxide into oxygen and hydrogen peroxide and is our primary anti-oxidant combating enzyme. SOD1 performs its function with the help of co-factors and one protein in particular is vital to its proper functioning. Copper chaperone protein (CCS) is a metalloprotein that specifically delivers Cu to SOD1 and may activate SOD1 through direct insertion of the Cu cofactor. Interestingly, defects in SOD1 are the cause of a form of ALS (Amyotrophic Lateral Sclerosis or Lou Gehrig′s disease) and much research is centered on understanding how over-active or under-active SOD1 contributes to the disease. EMD-Millipore′s Anti-SOD1 mouse monoclonal antibody has been tested in western blot on HeLa, NIH3T3, A549 and A431 cell lysates and fluorescent immunocytochemistry on PANC1, SKBR-3 and 3T3-L1 cells in culture. The antibody has also been tested in flow cytometry on A431 cells in culture.

免疫原

Purified recombinant fragment of human SOD1 expressed in E. Coli.

應用

Anti-SOD1 Antibody, clone 6F5 is a highly specific mouse monoclonal antibody, that targets Superoxide Dismutase & has been tested in western blotting, ICC & Flow Cytometry.

Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected SOD1 in PANC-1, SKBR-3, and 3T3-L1 cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SOD1 in non serum starved A431 cells.

Optimal working dilutions must be determined by end user.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

品質

Evaluated by Western Blotting in HeLa, NIH/3T3, A549, and A431 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SOD1 in HeLa, NIH/3T3, A549, and A431 cell lysates.

標靶描述

~18 kDa observed. Uncharacterized bands may appear in some lysate(s).

外觀

Unpurified

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
HeLa, NIH/3T3, A549, and A431 cell lysates

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable