MABC632

Anti-MAP3K5 Antibody, clone 2E4

ascites fluid, clone 2E4, from mouse

• 同義詞: Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, ASK-1, MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: ascites fluid
• 抗體產品種類: primary antibodies
• 無性繁殖: 2E4, monoclonal
• 物種活性: human
• 技術: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG1
• NCBI登錄號: NP_005914
• UniProt登錄號: Q99683
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MAP3K5(4217)

描述

一般說明

Mitogen-activated protein kinase kinase kinase 5 (MAP3K5), also known as, MAPK/ERK kinase 5 (MEK Kinase 5; MEKK 5) or Apoptosis signal-regulating kinase 1 (ASK-1) protein, is a serine/threonine kinase that is part of the MAP kinase signal transduction pathway. The MAP kinase pathway is central to several signaling cascades the help determine everything from cell fate in differentiation to cell survival in apoptosis. MAP3K5 is critical for innate immune response against numerous pathogens and MAP3K5 also mediates cells responses to various cellular stresses such as oxidative stress, and responses mediated by inflammatory signaling agents such as TNF or LPS. Moreover, MAP3K5 has has been found to be involved in cancer, diabetes, cardiovascular and neurodegenerative diseases. MAP3K5 is membrane associated though not membrane bound directly. Its expression thus is primarily endoplasmic reticulum but can become strongly perinuclear under some conditions. Found in most tissues, it is abundant in heart and pancreas. It′s activity is regulated via phosphorylation-dephosphorylation processes as well as though its interactions with a many other protein partners to form numerous multimolecular cell signaling complexes. EMD-Millipore’s Anti-MAP3K5 mouse monoclonal, clone 2E4, has been tested in western blotting against recombinant human MAP3K5 protein, and in paraffin-embedded immunohistochemistry with human cancer tissues, and in immunofluorescent immunocytochemistry, and flow cytometry on human HeLa cells in culture.

免疫原

Purified recombinant fragment of human MAP3K5 expressed in E. coli.

應用

Anti-MAP3K5 Antibody, clone 2E4 is a highly specific mouse monoclonal antibody, that targets MAP3K5 & has been tested in western blotting, IHC, ICC, Flow Cytometry & ELISA.

Immunohistochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected MAP3K5 in human pancreas, heart, breast cancer, and prostate tissues.

Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected MAP3K5 in HeLa cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected MAP3K5 in HeLa cells.

ELISA: A representative lot detected MAP3K5 in ELISA (Direct).

Optimal working dilutions must be determined by end user

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

品質

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MAP3K5 in 10 µg of HepG2 cell lysate.

標靶描述

~155 kDa observed. Uncharacterized bands may appear in some lysate(s).

外觀

Unpurified

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
HepG2 cell lysate

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable