MABF291

Anti-S100A8/S100A9 Antibody, clone 5.5

clone 5.5, from mouse

• 同義詞: Protein S100-A9, Calgranulin-B, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, S100 calcium-binding protein A9, AMRP-14, p14, Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fi
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 5.5, monoclonal
• 物種活性: human
• 技術: ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_002955; NP_002956
• UniProt登錄號: P05109; P06702
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... S100A8(6279) , S100A9(6280)

描述

一般說明

S100A8, also known as Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen (CFAG), Leukocyte L1 complex light chain, Migration inhibitory factor related protein 8 (MRP-8), p8, S100 calcium binding protein A8, or Urinary stone protein band A, and encoded by the gene S100A8/CAGA/CFAG/MRP8, is an important protein that binds both zinc and calcium and plays an important role in the regulation of inflammatory processes and immune response. Found in complex with its partner S100A9, S100A8 facilitates chemotaxis, fatty acid trafficking, cytoskeleton reorganization, and NAPDPH oxidase activation and other intracellular activities, as well as a host of extracellular induced activities such as extracellular proinflammatory, antimicrobial, oxidant scavenging, and apoptotic activities . Additionally S100A8 can act as a potent autoimmunity amplifier and appears to augment various cancers and their spread when over expressed. S100A8 is widely expressed and used as biomarker in patients with inflammatory diseases and as a cancer marker in multiple forms of cancer.

特異性

This antibody detects both S100A8 and S100A9.

免疫原

Whole cells expressing Human S100A8 & S100A9.

應用

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

This Anti-S100A8/S100A9 Antibody, clone 5.5 is validated for use in Flow Cytometry and Immunoprecipitation and Immunohistochemistry and ELISA and Western Blotting for the detection of S100A8/S100A9.

Western Blotting Analysis: A representative lot of this antibody was used to detect S100A8/S100A9 in Human monocyte and neutrophil extracts (Edgeworth, J., et al., (1991) JBC. 266(12):7706-7713).
Western Blotting Analysis: A representative lot of this antibody was used to detect S100A8/S100A9 in neutrophil extracts (Hogg et al., 1989).
Immunoprecipitation Analysis: A representative lot of this antibody was used to detect S100A8/S100A9 in Human monocyte and neutrophil lysate (Edgeworth, J., et al., (1991) JBC. 266(12):7706-7713).
Immunoprecipitation Analysis: A representative lot of this antibody was used to detect S100A8/S100A9 in MRP-8 and TL-14 mutant lysate (Hessian P.A., et al., (2001) Eur. J. Biochem. 268:353-363).
Immunohistochemistry Analysis: A representative lot of this antibody was used to detect S100A8/S100A9 in Human Bronchus tissue (Hogg, N., et al., (1989) Eur. J. Immunol. 19:1053-1061).
Immunohistochemistry Analysis: A representative lot of this antibody was used to detect S100A8/S100A9 in spleen and thymus tissue (Hogg, N., et al., (1989) Eur. J. Immunol. 19:1053-1061).
ELISA: A representative lot of this antibody was used to detect S100A8/S100A9 in ELISA (Ryckman, C., et al., (2003) Arthritis & Rheumatism. 48(8):2310-2320).

品質

Evaluated by Flow Cytometry on Human PBMCs.

Flow Cytometry Analysis: A 1:80 dilution (0.25 µg) of this antibody detected S100A8 and/or S100A9 in 1x10E6 PBMCs.

標靶描述

~8 kda(11kDa) and 14 kDa and as a heterodimer it would be ~24 kDa (under native conditions)

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable