MABC570

Anti-Sec8 Antibody/Exoc4, clone 2E12

clone 2E12, from mouse

• 同義詞: Exocyst complex component 4, Exocyst complex component Sec8, rSec8
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2E12, monoclonal
• 物種活性: human, rat, mouse
• 技術: immunocytochemistry: suitable; western blot: suitable
• 同型: IgG2bκ
• NCBI登錄號: NP_446327
• UniProt登錄號: Q62824
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... EXOC4(60412)

描述

一般說明

Sec8/Exoc4 is a component of the exocyst complex which is a multi-protein complex used for docking of exocytic vesicles with fusion sites on the plasma membrane. The exocyst complex is essential for exocytosis which is the process whereby proteins, hormones, transmitters etc. are transported and released to the extracellular environment. The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Sec8/Exoc4 appears to be important for tethering of secretory vesicles to specific regions on the plasma membrane. It seems particularly important in normal development for growth and morphological change by allowing the secretion of matrix metalloproteinases such as MMP2 or MMP9 in discrete locations. Knockdowns exhibit reduced cellular proliferation as well as invasive activities as well. As such Sec8/Exoc4 is important in tumor growth and invasion as well, thus it is also a current target for cancer therapy.

免疫原

Recombinant protein corresponding to rat Sec8/Exoc4.

應用

Immunocytochemistry Analysis: A 1:50-250 dilution from a representative lot detected Sec8/Exoc4 in HeLa, A431, and NIH/3T3 cells. DyLight^® is a registered trademark of Thermo Fisher Scientific.
Western Blotting Analysis: A representative lot detected Sec8/Exoc4 in HeLa cells treated with EXO70 SiRNA (Liu, J., et al. (2007). Molecular Biol. Cell. 18:4483-4492).
Western Blotting Analysis: A representative lot detected Sec8/Exoc4 in B16F1 cells treated with siRNA targeting Sec8 (Zhao, Y., et al. (2013). Dev. Cell. 26:266-278).
Immunocytochemistry Analysis: A representative lot detected Sec8/Exoc4 in HeLa cells transfected with GFP-Exo70 (Liu, J., et al. (2007). Molecular Biol. Cell. 18:4483-4492).
Immunocytochemistry Analysis: A representative lot detected Sec8/Exoc4 in Rat emreyonic hippocampal neurons (Menchon, S.A., et al. (2011). PLOS ONE. 6(9):e24190 1-11).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Sec8 Antibody/Exoc4, clone 2E12 is validated for use in Western Blotting and Immunocytochemistry for the detection of Sec8/Exoc4.

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Sec8/Exoc4 in 10 µg of HeLa cell lysate.

標靶描述

~110 kDa observed

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

DyLight is a registered trademark of Pierce Biotechnology, Inc.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable