MABC286

Anti-Tankyrase-1/TNKS Antibody, clone 7D1.2

clone 7D1.2, from mouse

• 同義詞: Tankyrase-1, TANK1, ADP-ribosyltransferase diphtheria toxin-like 5, ARTD5, Poly [ADP-ribose] polymerase 5A, TNKS-1, TRF1-interacting ankyrin-related ADP-ribose polymerase, Tankyrase I
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 7D1.2, monoclonal
• 物種活性: mouse, human
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG3κ
• NCBI登錄號: NP_003738
• UniProt登錄號: O95271
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... TNKS(8658)

描述

一般說明

Tankyrase-1/TNKS is a critical enzyme in Wnt signaling pathway. Tankyrase-1/TNKS is a poly-ADP-ribosyltransferase which is a key mediator of PARsylation: Becoming ADP-ribosylated. PARsylation is important in Wnt signaling because ADP-ribosylation of AXIN1 and AXIN2, two key components of the beta catenin destruction complex, is necessary for destruction via RNF146 (an ubiquitin lyase) and ubiquitination and subsequent destruction of beta catenin. Tankyrase-1/TNKS also mediates PARsylation of TERF1 and thus is important in controlling telomere length, and Tankyrase-1/TNKS’s PARsylation of other proteins makes Tankyrase-1/TNKS important in vesicle trafficking and subcellular protein distribution as well. Tankyrase-1/TNKS is widely expressed, and is found mostly cytoplasmically but can be associated with the nucleus and spindles and Golgi complexes as well.

免疫原

GST-tagged recombinant protein corresponding to Human Tankyrase-1/TNKS.

應用

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Tankyrase-1/TNKS in Hela, A431, and N1H/3T3 cells.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Tankyrase-1/TNKS in neurons and glial cells in human cerebral cortex tissue, Purkinje, basket and granule cells in human cerebellum tissue, and neurons in human thalamus tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Tankyrase-1/TNKS Antibody, clone 7D1.2 is validated for use in Western Blotting and Immunocytochemistry and Immunohistochemistry for the detection of Tankyrase-1/TNKS.

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected Tankyrase-1/TNKS in 10 µg of HeLa cell lysate.

標靶描述

~142 kDa observed.
Uncharacterized bands may be observed in some lysates.

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable