MABC14

Anti-VHL Antibody, clone 11E12

clone 11E12, from mouse

• 同義詞: von Hippel-Lindau disease tumor suppressor, Protein G7, elongin binding protein, von Hippel-Lindau syndrome, pVHL
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 11E12, monoclonal
• 物種活性: human, mouse
• 技術: immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_000542
• UniProt登錄號: P40337
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... VHL(7428)

描述

一般說明

von Hippel-Lindau protein (VHL) is most widely recognized as belonging to an E3 ubiquitin ligase complex that specifically targets hypoxia-inducible factors (HIFs) for the purpose of polyubiquitination and proteolytic degradation. Within this complex, VHL acts as a target recruitment subunit for HIFα and its subsequent ubiquitination. The complex also contains proteins such as B and C elongins, Rbx1 and Cul2. Inactivation of VHL has been linked to renal cell carcinoma development, therefore suggesting that VHL plays a role as a tumor suppressor. VHL expression has been observed in both fetal and adult kidney and brain tissues. Defects in VHL expression are causal to renal cell carcinoma type 1 (RCC1), erythrocytosis familial type 2 (ECYT2), von Hippel-Lindau disease (VHLD), and pheochromocytoma.

免疫原

Purified human VHL

應用

Immunoprecipitation: It has been shown that this VHL clone will immunoprecipitate VHL from HEK 293 cells (Shoenfeld, 1998).

Research Category
Apoptosis & Cancer

Signaling

Research Sub Category
Tumor Markers

Ubiquitin & Ubiquitin Metabolism

This Anti-VHL Antibody, clone 11E12 is validated for use in WB, IP for the detection of VHL.

品質

Evaluated by Western Blot in mouse fetal brain tissue lysate.

Western Blot Analysis: 1 µg/mL of the antibody detected VHL in 10 µg of mouse fetal brain tissue lysate.

標靶描述

~ 24 kDa observed MW. VHL SUMOylation may result in high MW bands (Cai, 2010).

外觀

Protein G

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Mouse fetal brain tissue lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable