MAB3369

Anti-MMP-3 Antibody, clone SL-1 IID4

clone SL-1 IID4, Chemicon^®, from mouse

• 同義詞: Stromelysin-1, Transin, Proteoglycanase
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: SL-1 IID4, monoclonal
• 物種活性: human
• 製造商／商標名: Chemicon^®
• 技術: ELISA: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG2b
• NCBI登錄號: NM_002422.3
• UniProt登錄號: P08254
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MMP3(4314)

描述

一般說明

MMPs are frequently expressed in malignant neoplastic cells and play crucial roles in tumor invasion and metastasis. Tissue inhibitors of matrix metalloproteinases (TIMPs) control the activitiy of MMPS. The tissue distribution of three major MMPs has been defined in human mammary pathology: 1) collagenase (MMP-1) which degrades fibrillar interstitial collagens; 2) a 72-k/Da gelatinase (MMP-2) which mainly degrades type IV collagen and denatured collagens; and 3) stromelysin (MMP-3) which has a wider range of action, degrading several matrix components including the core proteins of proteoglycans, laminin and non-helical regions of collagens.

特異性

MAB3369 recognizes one doublet of 54kDa/59kDa and another doublet of 44kDa/49kDa, which are identified as unglycosylated and glycosylated species of pro (latent) and active forms of matrix metalloproteinase-3 (MMP-3; also known as Stromelysin-1 of Transin). MAB3369 shows no cross-reaction with the pro and active forms of other MMPs.

CELLULAR LOCALIZATION:

Cytoplasmic

免疫原

APMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1

應用

Anti-MMP-3 Antibody, clone SL-1 IID4 is an antibody against MMP-3 for use in ELISA, IP, WB, IH(P).

Immunofluorescence

Immunoprecipitation (Use Protein A): 2μg/ml protein lysate.

Western blotting: 0.5-1.0 μg/ml for 2hrs at RT

Immunohistology (frozen & formalin-fixed): 10-20 μg/ml for 60 minute at room temperature. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissue sections.

Optimal working dilutions and protocols must be determined by end user.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

外觀

Format: Purified

Purified from ascites fluid by Protein A chromatography. 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

儲存和穩定性

Antibody with sodium azide is stable for 12 months when stored at 2-8°C.

分析報告

Control
POSTIVE CONTROL:

Human endometrium cells, placenta, bladder, breast, ovarian carcinomas.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable