MABF550

Anti-CD25 Antibody (mouse), PerCP-Cy5.5, clone PC61.5

clone PC61.5, 0.2 mg/mL, from rat

• 同義詞: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55, CD25
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.44

屬性

• 生物源: rat
• 品質等級: 100
• 共軛: PerCP-Cy5.5
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: PC61.5, monoclonal
• 物種活性: mouse
• 包裝: antibody small pack of 25 μg
• 濃度: 0.2 mg/mL
• 技術: flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• 同型: IgG1λ
• UniProt登錄號: P01590
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Il2Ra(16184)

描述

一般說明

The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells.
The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-.

應用

This rat monoclonal antibody Anti-CD25 (mouse), PerCP-Cy5.5, clone PC61.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD25.

品質

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD25 in one million C57Bl/6 splenocytes.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable