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一般說明
Cadherins are a family of transmembrane glycoproteins involved in Ca2+-dependent cell to cell interactions. The members of the family are differentially expressed in various tissues and function in the maintenance of tissue integrity, morphogenesis, and migration. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), placental cadherin (P-cadherin) and retinal cadherin (R-cadherin). Type II include kidney cadherin (K-cadherin) and osteoblast cadherin (OB-cadherin). One of the best characterized cadherins is E-cadherin (epithelial cadherin or cadherin-1). It consists of calcium binding extracellular domains responsible for interactions with other E-cadherin molecules on neighboring cells and interaction with integrin alphaEbeta7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.
免疫原
Whole cells corresponding to human E-Cadherin.
應用
Flow Cytometry Analysis: 2 µg from a representative lot detected E-Cadherin in 1X10E6 A431 cells.
Flow Cytometry Analysis: A representative lot from an independent laboratory detected E-Cadherin in bone marrow cells (Burhing, H. J., et al. (1996). Leukemia. 10(1):106-116.)
Inhibition Analysis: A representative lot from an independent laboratory inhibited E-Cadherin-mediated adhesion of Langerhans to keratinocytes (Caberg, J. H., et al. (2008). Carcinogenesis. 29(7):1441-1447.).
Inhibition Analysis: A representative lot from an independent laboratory inhibits human embryoid body agglomeration (Dang, S. M., et al. (2004). Stem Cells. 22: 275-282.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected E-Cadherin in human thymus tissue (Kutlesa, S., et al. (2002). J Cell Sci. 115(Pt. 23):4505-4515.).
Western Blotting Analysis: A representative lot from an independent laboratory detected E-Cadherin in human thymus tissue lysate (Kutlesa, S., et al. (2002). J Cell Sci. 115(Pt. 23):4505-4515.).
Flow Cytometry Analysis: A representative lot from an independent laboratory detected E-Cadherin in bone marrow cells (Burhing, H. J., et al. (1996). Leukemia. 10(1):106-116.)
Inhibition Analysis: A representative lot from an independent laboratory inhibited E-Cadherin-mediated adhesion of Langerhans to keratinocytes (Caberg, J. H., et al. (2008). Carcinogenesis. 29(7):1441-1447.).
Inhibition Analysis: A representative lot from an independent laboratory inhibits human embryoid body agglomeration (Dang, S. M., et al. (2004). Stem Cells. 22: 275-282.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected E-Cadherin in human thymus tissue (Kutlesa, S., et al. (2002). J Cell Sci. 115(Pt. 23):4505-4515.).
Western Blotting Analysis: A representative lot from an independent laboratory detected E-Cadherin in human thymus tissue lysate (Kutlesa, S., et al. (2002). J Cell Sci. 115(Pt. 23):4505-4515.).
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-E-Cadherin Antibody, clone 67A4, Azide & Ascites Free is validated for use in western blotting, flow cytometry, inhibition & immunofluorescence for the detection of E-Cadherin.
品質
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected E-Cadherin in 10 µg of A431 cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected E-Cadherin in 10 µg of A431 cell lysate.
標靶描述
~97 kDa observed
聯結
Replaces: 04-1103
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
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