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Key Documents

MAB3101

Sigma-Aldrich

Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 5B9.2

clone 5B9.2, Chemicon®, from mouse

同義詞:

Cx45

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

5B9.2, monoclonal

物種活性

rat, rabbit, human

製造商/商標名

Chemicon®

技術

immunocytochemistry: suitable
western blot: suitable

同型

IgG1

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... GJC1(10052)
rat ... Gjc1(266706)

特異性

Recognizes Human Connexin 45.

SPECIES REACTIVITIES:

Due to sequence homologies, this antibody is also expected to react with canine (100% homology) and mouse (92% homology) Connexin 45. Also confirmed to work on rat C6 cells. Others not tested.

免疫原

Epitope: near C-terminus, cytoplasmic
The peptide sequence used for the immunogen was H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu-Cys-OH conjugated to KLH, corresponding to amino acids 354-367 of human connexin 45.

應用

Anti-Connexin 45 Antibody, near C-terminus, cytoplasmic, clone 5B9.2 detects level of Connexin 45 & has been published & validated for use in IC & WB.
Immunblotting (~46 kDa)

Immunocytochemistry

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

外觀

Format: Purified
Liquid at 1 mg/mL in 0.02M PB with 0.25 M NaCl, pH=7.6 containing 0.1% sodium azide.

儲存和穩定性

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Expression of connexins 36, 43, and 45 during postnatal development of the mouse retina.
Alexandre Hiroaki Kihara, Leandro Mantovani de Castro, Monica Aparecida Belmonte et al.
Journal of Neurobiology null
Connexin45-containing neuronal gap junctions in rodent retina also contain connexin36 in both apposing hemiplaques, forming bihomotypic gap junctions, with scaffolding contributed by zonula occludens-1.
Li, X; Kamasawa, N; Ciolofan, C; Olson, CO; Lu, S; Davidson, KG; Yasumura, T; Shigemoto et al.
The Journal of Neuroscience null
Axonal synapses utilize multiple synaptic ribbons in the mammalian retina.
Kim, HL; Jeon, JH; Koo, TH; Lee, UY; Jeong, E; Chun, MH; Moon, JI; Massey, SC; Kim, IB
Testing null
Electrical synapses in retinal ON cone bipolar cells: subtype-specific expression of connexins.
Han, Y; Massey, SC
Proceedings of the National Academy of Sciences of the USA null
Alexandre H Kihara et al.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 28(1), 39-52 (2009-10-06)
Connexin (Cx) channels and hemichannels are involved in essential processes during nervous system development such as apoptosis, propagation of spontaneous activity and interkinetic nuclear movement. In the first part of this study, we extensively characterized Cx gene and protein expression

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