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Key Documents

MAB201C

Sigma-Aldrich

Mouse Anti-Rabbit light chain Antibody, Cy3 conjugate

Chemicon®, from mouse

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

共軛

CY3 conjugate

抗體產品種類

secondary antibodies

無性繁殖

monoclonal

物種活性

rabbit

製造商/商標名

Chemicon®

技術

ELISA: suitable
western blot: suitable

同型

IgG

運輸包裝

wet ice

目標翻譯後修改

unmodified

一般說明

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

特異性

Minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep.
The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rabbit IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep immunoglobulins.

免疫原

Epitope: Kappa light chain
Prepared from rabbit IgG light chain.

應用

Mouse anti-Rabbit light chain Antibody, Cy3 conjugate detects level of Rabbit light chain & has been published & validated for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Western Blotting: 1:100 -1:800
ELISA: 1:100 -1:800

Optimal working dilutions must be determined by the end user.

標靶描述

25 kDa

外觀

Ascites
Isolated from ascites by chromatographic procedures. Liquid in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer.

儲存和穩定性

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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