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Key Documents

MAB1911

Sigma-Aldrich

Anti-Tenascin Antibody, type III repeats 1-3, clone 9F8

clone 9F8, Chemicon®, from mouse

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

9F8, monoclonal

物種活性

human

製造商/商標名

Chemicon®

技術

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

同型

IgG1κ

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... TNC(3371)

特異性

Binds to a recombinant fragment of human tenascin containing the fibronectin type III repeats, numbers 1-3.

免疫原

Epitope: type III repeats 1-3
Purified human tenascin

應用

Immunohistochemistry at 2 μg/mL on tissues fixed with cold acetone

Immunocytochemistry at 2 μg/mL on U251 cells

Immunoblotting (Western) at 2 μg/mL to detect whole tenascin under reducing conditions

ELISA: 2 x 10-10 M endpoint dilution

Affinity constant Kd= 6.37 x 10-9

Final working dilutions must be determined by end user.
This Anti-Tenascin Antibody, type III repeats 1-3, clone 9F8 is validated for use in ELISA, WB, IC, IH for the detection of Tenascin.

外觀

Format: Purified
Liquid in 0.02 M phosphate, 0.25 M Sodium Chloride pH 7.6, with 0.1% Sodium Azide.

儲存和穩定性

Store at 2 and 8°C for up to 6 months.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Wing S To et al.
The Journal of biological chemistry, 286(17), 14881-14891 (2011-02-18)
Interactions between fibronectin and tenascin-C within the extracellular matrix provide specific environmental cues that dictate tissue structure and cell function. The major binding site for fibronectin lies within the fibronectin type III-like repeats (TNfn) of tenascin-C. Here, we systematically screened

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