MAB131884

Anti-BASE-tag Antibody, clone 7C7

clone 7C7, from mouse

• 同義詞: Capsid protein VP1, Capsid protein AVV9
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.43
• 無性繁殖: 7C7, monoclonal
• application: IF; IP; WB
• 物種活性: adenovirus-infected cells, virus
• 技術: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable

屬性

• 生物源: mouse
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 7C7, monoclonal
• 物種活性: adenovirus-infected cells, virus
• 包裝: antibody small pack of 25 μL
• 技術: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG2bκ
• UniProt登錄號: Q6JC40
• 目標翻譯後修改: unmodified

描述

一般說明

Capsid protein VP1 (UniProt: Q6JC40) is encoded by the Cap gene in Adeno-associated virus 9 (AAV9). Adeno-associated viruses (AAV) are small non-enveloped DNA viruses that can serve as promising viral vectors for gene delivery, but can also elicits cellular and humoral immune responses. AAVs are reported to be nonpathogenic in humans and animals and display low immunogenicity. Following their attachment to cell surface receptor they are internalized via endocytosis and their intracellular trafficking is a rate-limiting, slow and inefficient process. AAVs contain an icosahedral capsid, which is 22 to 26 nm in diameter. They are shown to have a single-stranded DNA genome of approximately 4.7 kilobases that is flanked by two T-shaped inverted terminal repeats (ITRs). The 7C7 an epitope-tag antibody recognizes a basic ten amino acid epitope (QPAKKRLNFGQTGD) with high specificity. The basic nature of the BASE-tag epitope sequence distinguishes it from other commonly used epitope tags, such as Myc, EQKLISEEDL, FLAG, DYKDDDDK, HA, YPYDVPDYA, V5 etc. Clone 7C7 recognizes capsid proteins VP1 and VP2, but not VP3. Based on sequence identity of the epitope sequence, this antibody is expected to recognize VP1 and VP2 of AAV serotypes 1,2,3,6,7,8, and 9. (Ref.: Nonnenmacher, M., and Weber, T (2012). Gene Ther. 19(6); 649-658).

特異性

Clone 7C7 is a mouse monoclonal antibody that detects various serotypes of Adeno-associated virus (AVV). It targets an epitope with in 14 amino acids from the N-terminal region.

免疫原

Empty Adeno-associated virus 9 (AAV9) Capsids

應用

Anti-BASE-tag, clone 7C7, Cat. No. MAB131884, is a mouse monoclonal antibody that recognizes the epitope QPAKKRLNFGQTGD and has been tested for use in Western Blotting, Immunoprecipitation, and Immunofluorescence.

Immunoprecipitation: A representative lot immunoprecipitated EGFP-BASE-tag-Actin in HEK293T cells transfected with EGFP-BASE-tag-Actin (Courtesy: Dr. Thomas Weber, Icahn School of Medicine at Mount Sinai, New York).
Immunofluorescence: A representative lot detected EGFP-BASE-tag-Actin in HeLa cells transfected with EGFP-BASE-tag-Actin (Courtesy: Dr. Thomas Weber, Icahn School of Medicine at Mount Sinai, New York).

Research Category
Inflammation & Immunology

品質

Evaluated by Western Blotting in HEK293 cells transfected with GFP-7C7-Actin.

Western Blotting Analysis: A 1:500 dilution of this antibody detected BASE-tag in HEK293 cells transfected with GFP-7C7-Actin.

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.