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Merck
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重要文件

IP05

Millipore

蛋白质G Plus /蛋白质A琼脂糖悬浮液

Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

同義詞:

Affinity resin, Protein G/Agarose

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About This Item

分類程式碼代碼:
41116133
NACRES:
NA.56

形狀

slurry (Liquid)

包含

≤0.1% sodium azide as preservative

製造商/商標名

Calbiochem®

儲存條件

do not freeze

技術

protein purification: suitable

適合性

suitable for microbiology

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

专为免疫沉淀应用而设计。该产品已使用 BSA 进行封闭,以减少非特异性结合,不能用于纯化。
专门配制用于免疫沉淀的蛋白 G PLUS/蛋白 A 琼脂糖混合物。

應用

免疫沉淀法(参见评论)

警告

毒性:标准处理(A)

外觀

PBS 中 33% 的浆液。

其他說明

提供琼脂糖溶液以备使用。蛋白 G Plus/蛋白 A 琼脂糖免疫沉淀试剂被 BSA 封闭,不应用于免疫球蛋白纯化或共价交联。对于免疫沉淀反应,建议每 µg 一抗加入 15 µl 溶液。预清除将最大程度地减少非特异性沉淀导致的多余条带。要进行预清除,将与免疫沉淀抗体相同物种的 20 µl 琼脂糖结合物和 1 µg 正常 IgG 加入样品中。当使用免疫印迹进行检测时,某些二抗可以与 BSA 或样品中存在的高浓度的其他蛋白质发生非特异性反应。这可以通过降低二抗的浓度来消除。

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Journal of neuroinflammation, 18(1), 97-97 (2021-04-22)
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Journal of virology, 73(12), 9917-9927 (1999-11-13)
Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent for adult T-cell leukemia/lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis. Tax(1) is a 40-kDa phosphoprotein, predominantly localized in the nucleus of the host cell, which functions to transactivate both
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Pathology international, 69(7), 381-391 (2019-07-11)
Cten is an oncogene promoting EMT in many signaling pathways, namely through Snail. We investigated whether Cten function could be mediated through Src. Cten levels were modulated by forced expression in HCT116 and gene knockdown in SW620 CRC (colorectal cancer)
Bin Tang et al.
Disease markers, 2022, 9087190-9087190 (2022-04-23)
To explore the regulatory effect of ubiquitin specific protease 25 (USP25) on glioma cell proliferation, migration, invasion, and its underlying mechanism. The USP25-overexpressed and USP25-knockdown glioma cells were established on U251 and U87 cells, respectively. Glioma cell proliferation ability was

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