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Merck
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Key Documents

IP04

Millipore

蛋白质G Plus-琼脂糖悬浮液

Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.

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About This Item

分類程式碼代碼:
41116133
NACRES:
NA.56

形狀

slurry (Liquid)

包含

≤0.1% sodium azide as preservative

製造商/商標名

Calbiochem®

儲存條件

do not freeze

技術

immunoprecipitation (IP): suitable

適合性

suitable for immunoprecipitation

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

专为免疫沉淀应用而设计。本品用BSA阻断,以减少非特异性结合,不能用于纯化;最适合牛,山羊,人和大鼠IgG和小鼠IgG1
蛋白G PLUS琼脂糖混悬剂,专门配制用于免疫沉淀。

警告

毒性:标准处理(A)

其他說明

提供琼脂糖溶液以备使用。蛋白G Plus琼脂糖被BSA阻断,不应用于免疫球蛋白纯化或共价交联。对于免疫沉淀反应,建议使用15 µl/µg一抗。预清除将最大程度地减少非特异性沉淀导致的多余条带。要进行预清除,将与免疫沉淀抗体相同物种的 20 µl 琼脂糖结合物和 1 µg 正常 IgG 加入样品中。当使用免疫印迹进行检测时,某些二抗可以与 BSA 或样品中存在的高浓度的其他蛋白质发生非特异性反应。这可以通过降低二抗的浓度来消除。

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Leo K Iwai et al.
Journal of proteome research, 9(6), 3135-3145 (2010-05-05)
Type 1 diabetes, in human patients and NOD mice, results from an immune attack on insulin-producing beta-cells of the pancreas by autoreactive T lymphocytes. In NOD mice, genetically controlled perturbations in the signaling pathways downstream of the antigen-specific T cell
Hayeong Kwon et al.
Journal of cellular and molecular medicine, 13(8A), 1549-1564 (2009-09-26)
Mitogenic regulation by caveolin-2 in response to insulin was investigated. Insulin triggered phosphorylation of caveolin-2 on tyrosine 19. Insulin increased the interaction between pY19-caveolin-2 and phospho-ERK, and that interaction was inhibited by a MEK inhibitor U0126. Insulin-induced interaction of caveolin-2
Kensuke Sakamoto et al.
Molecular biology of the cell, 21(16), 2966-2974 (2010-06-25)
CREB (cyclic AMP response element-binding protein) is a stimulus-induced transcription factor that plays pivotal roles in cell survival and proliferation. The transactivation function of CREB is primarily regulated through Ser-133 phosphorylation by cAMP-dependent protein kinase A (PKA) and related kinases.
Siqi Li et al.
The Journal of pathology, 254(3), 265-278 (2021-04-03)
Chemoresistance is a major obstacle to the treatment of triple-negative breast cancer (TNBC), which has a poor prognosis. Increasing evidence has demonstrated the essential role of cancer stem cells (CSCs) in the process of TNBC chemoresistance. However, the underlying mechanism
Roshia Ali et al.
Frontiers in oncology, 12, 841303-841303 (2022-03-12)
SNTA1 signaling axis plays an essential role in cytoskeletal organization and is also implicated in breast cancers. In this study, we aimed to investigate the involvement of actin cytoskeleton in the propagation of SNTA1/p66shc mediated pro-metastatic cascade in breast cancer

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