FCMAB116P

Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70

clone MC-813-70, Milli-Mark^®, from mouse

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 共軛: PE
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: MC-813-70, monoclonal
• 物種活性: human
• 製造商／商標名: Milli-Mark^®
• 技術: flow cytometry: suitable; immunocytochemistry: suitable
• 同型: IgG3κ
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... FUT4(2526)

描述

一般說明

N-terminal GST-tagged, recombinant human MuRF1 full length, expressed in E.coli. Purified using glutathione sepharose.

The cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.

特異性

This antibody reacts with the stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES).
No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In ctontrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.

免疫原

Human embryonal carcinoma cell line 2102Ep.

應用

Immunocytochemical staining of live H9 human ES cells incubated for 30 minutes at 37oC with 1:50 dilution of anti-SSEA-4, clone MC-813-70, PE conjugate (Cat. No. FCMAB116P) monoclonal antibody.
Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Immunocytochemical staining of fixed H9 human ES cells incubated for 2 hours at 2-8oC with 1:50 dilution of anti-SSEA-4, clone MC-813-70, PE conjugated (Cat. No. FCMAB116P) monoclonal antibody.
Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70 is validated for use in FC, IC for the detection of SSEA-4.

外觀

Protein A purified

Purified mouse monoclonal IgG3 conjugated to PE fluorochrome in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.

儲存和穩定性

Maintain refrigerated at 2-8oC protected from light in undiluted aliquots for up to 6 months from date of receipt.

分析報告

Control
Pluripotent human embryonic stem (ES) cells

法律資訊

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable