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Key Documents

DC03L

Sigma-Aldrich

山羊抗兔IgG过氧化物酶偶联物

lyophilized, Calbiochem®

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About This Item

分類程式碼代碼:
12352203

生物源

goat

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

lyophilized

不包含

preservative

製造商/商標名

Calbiochem®

儲存條件

OK to freeze

運輸包裝

ambient

儲存溫度

2-8°C

目標翻譯後修改

unmodified

相關類別

一般說明

免疫亲和纯化的山羊多克隆抗体,吸附人血清蛋白并与辣根过氧化物酶偶联。可识别兔IgG。
经验证,该山羊抗兔IgG过氧化物酶偶联物可用于ELISA和免疫组化以检测兔IgG。

應用

ELISA(1:5000-1:100,000)

免疫印迹(1:5000-1:100,000,比色;1:10,000-1:200,000,化学发光)

免疫组织化学(1:500-1:5000)

警告

毒性:标准处理(A)

外觀

从250 mM NaCl,10 mM磷酸钠,15 mg/ml BSA(pH 7.6)中冻干。

重構

用1.5 ml H₂O复溶抗体。由于充分混合会使抗体变性,因此在4°C下轻轻混合使其溶解。在复溶后分装并冷冻保存(-70°C)。避免溶液反复冻融。或者,可以添加甘油(最终浓度为50%),然后将复溶的抗体储存于-20°C。请注意,在这些条件下,抗体和缓冲盐的浓度将为原始浓度的一半。

其他說明

为了确保这些试剂的质量,每个批次都使用我们的几种一抗进行测试。通过免疫电泳检测抗体特异性,结果显示对兔IgG和大多数兔免疫球蛋白常见的轻链具有特异性。未检测到与非免疫球蛋白血清蛋白的交叉反应性。该抗体也已通过ELISA和/或吸附进行了检测,以确保与人血清蛋白的交叉反应性降至最低,但该抗体可能与其他物种的免疫球蛋白具有交叉反应性。在单个系统中,应对抗体进行滴定以获得最佳结果。

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3


分析證明 (COA)

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