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Key Documents

CP01

Sigma-Aldrich

Anti-Actin (Ab-1) Mouse mAb (JLA20)

liquid, clone JLA20, Calbiochem®

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.43

生物源

mouse

品質等級

抗體表格

ascites fluid

抗體產品種類

primary antibodies

無性繁殖

JLA20, monoclonal

形狀

liquid

包含

≤0.1% sodium azide as preservative (actin antibody only, CP01-100UL)

物種活性(以同源性預測)

all

製造商/商標名

Calbiochem®

儲存條件

OK to freeze

同型

IgM

運輸包裝

wet ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... ACTB(60)

一般說明

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.
Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.
  • Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1
  • Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN
  • Entrez Gene Name: actin, alpha 1, skeletal muscle
  • Hu Entrez ID: 58, 71 (Related Antibodies: CP64)
  • Mu Entrez ID: 11459 ;-; 11465
  • Rat Entrez ID: 29437 ;-; 287876
  • 免疫原

    Chicken
    chicken cytoskeletal proteins

    應用

    Immunoblotting (1:5000-1:20000)

    Immunofluorescence (1:10000, see application references)



    Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)

    警告

    Toxicity: Multiple Toxicity Values, refer to MSDS (O)

    外觀

    100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.

    重構

    Following initial thaw, aliquot ascites and freeze (-20°C).

    分析報告

    Positive Control
    All animal cell lines

    其他說明

    Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
    Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
    Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
    Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
    Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
    Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

    法律資訊

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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    儲存類別代碼

    11 - Combustible Solids

    水污染物質分類(WGK)

    WGK 1

    閃點(°F)

    Not applicable

    閃點(°C)

    Not applicable


    分析證明 (COA)

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