跳轉至內容
Merck
全部照片(1)

Key Documents

16-157

Sigma-Aldrich

蛋白质A琼脂糖/鲑鱼精子DNA,2.5 mL

for use in chromatin immunoprecipitations (ChIP assays)

同義詞:

ChIP agarose beads, ChIP agaraose A beads, ChIP assays

登入查看組織和合約定價


About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.32

生物源

Staphylococcus aureus

品質等級

形狀

liquid

製造商/商標名

Upstate®

技術

ChIP: suitable

適合性

suitable for immunoprecipitation

運輸包裝

wet ice

一般說明

与含有经超声处理的鲑鱼精子DNA的琼脂糖通过烷基胺键共价结合的蛋白A。 鲑鱼精子DNA可封闭与染色质IP(ChIP)分析用微珠的非特异性相互作用。请注意ChIP试剂盒中的组分号16-157C不可单独订购,请购买16-157。
来自金黄色葡萄球菌的蛋白A对于IgG分子的Fc区具有非常高的亲和力。将蛋白A固定在微珠上会形成一种亲和树脂,可用于从粗血清、腹水或细胞培养基中纯化IgG组分。

應用

适合用于染色质免疫沉淀(ChIP)分析

品質

在染色质免疫沉淀中通过拉下与乙酰化组蛋白交联的DNA而进行常规评估; 后续的检测是采用ChIPs级(06-866)抗-乙酰化组蛋白H4抗体进行的

外觀

含有0.05%叠氮化钠的10mM Tris-HCl, 1mM EDTA, pH 8.0溶液。

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Protein Kinase C{zeta} Mediates Cigarette Smoke/Aldehyde- and Lipopolysaccharide-induced Lung Inflammation and Histone Modifications.
Yao H, Hwang JW, Moscat J, Diaz-Meco MT, Leitges M, Kishore N, Li X, Rahman I
The Journal of Biological Chemistry null
Methods for analyzing histone citrullination in chromatin structure and gene regulation.
Pingxin Li,Jing Hu,Yanming Wang
Methods in Molecular Biology null
Mohammad Kamran et al.
Journal of immunology (Baltimore, Md. : 1950), 205(12), 3311-3318 (2020-11-15)
IL-13 plays a critical role in mediating many biological processes responsible for allergic inflammation. Mast cells express Il13 mRNA and produce IL-13 protein in response to antigenic stimulation. Enhancers are essential in promoting gene transcription and are thought to activate
Activator-binding domains of the SWI/SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo.
Monica E Ferreira, Philippe Prochasson, Kurt D Berndt, Jerry L Workman, Anthony P H Wright
FEBS Journal null
Chromatin immunoprecipitation (ChIP) to assay dynamic histone modification in activated gene expression in human cells.
Lauren J Buro,Shaili Shah,Melissa A Henriksen
Journal of Visualized Experiments null

相關內容

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務