跳轉至內容
Merck
全部照片(5)

重要文件

檢視所有文件

CHP2NC

Sigma-Aldrich

Imprint® Ultra染色质免疫沉淀试剂盒，不含对照品

ChIP kit for maximum sensitivity, compatible with Next-Gen sequencing

登入查看組織和合約定價
全部照片(5)

About This Item

分類程式碼代碼:
41116158
NACRES:
NA.54

品質等級

200

一般說明

Imprint Ultra Chromatin IP 试剂盒是 Sigma′第二代染色质免疫沉淀（ChIP） 试剂盒，旨在实现最高灵敏度和最佳下一代测序结果。它提供了一套完整的染色质免疫沉淀解决方案，包括用于 DNA 纯化的色谱柱和试剂。该试剂盒让研究人员可以探索低丰度转录因子（TF） 的全基因组结合位点，以及新的组蛋白修饰。它针对来自 106 个细胞（最多约 50 μg DNA）的染色质的 ChIP 反应进行了优化，也可以扩大规模（或合并多个制剂）以适用于108 个细胞，用于 ChIP-chip 和 ChIP-Seq 应用中的全基因组结合研究。

應用

  • 适用于下游应用
  • 从单个靶标表征到全基因组分析的技术
  • 信号转导途径的表征
  • ChIp-chIP和ChIP-seq数据的验证

特點和優勢

  • 成功采用与低丰度、中等和高表达转录因子以及组蛋白修饰相关的 CHiP′ed DNA。
  • 更大的容量 - 可用于 2-10 × 106 的各种细胞数目范围
  • 最高的灵敏度 - 能够检测低至 2 × 106个细胞的低丰度转录因子
  • 采用 DNA 封闭的 "Staph-Seq" 进行IP（免疫沉淀），最大限度减少下游 ChIP-Seq 应用中的葡萄球菌A DNA 污染。

法律資訊

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

僅套裝組件

產品號碼
描述
  • Sodium dodecyl sulfate solution, for molecular biology, 10% in 18 megohm water
  • Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution

套裝中的組件也可單獨購買

產品號碼
描述
SDS
  • S6576ChIP Next Gen Seq Sepharose™SDS
  • I8896IGEPAL® CA-630, for molecular biologySDS
  • S5150Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell cultureSDS
  • W4502Water, Nuclease-Free Water, for Molecular BiologySDS
  • I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderSDS
  • I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powderSDS
  • A7638Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)SDS
  • A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)SDS
  • D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridizationSDS
  • M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solutionSDS
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDS
  • R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)SDS
查看全部 (12)

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

標靶器官

Central nervous system

儲存類別代碼

5.1A - Strongly oxidizing hazardous materials

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如果您需要協助，請聯絡 客戶支援

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Comparison of sample preparation methods for ChIP-chip assays.
O'Geen, H., et al.
Biotechniques, 41, 557-557 (2006)
Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions.
Chu, C., et al.
Molecular Cell (2011)
Philippe Collas
Molecular biotechnology, 45(1), 87-100 (2010-01-16)
The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest
Chromatin isolation by RNA purification (ChIRP).
Chu, C., et al.
Journal of Visualized Experiments (2012)
M H Kuo et al.
Methods (San Diego, Calif.), 19(3), 425-433 (1999-12-02)
Chromatin structure plays important roles in regulating many DNA-templated processes, such as transcription, replication, and recombination. Considerable progress has recently been made in the identification of large, multisubunit complexes dedicated to these nuclear processes, all of which occur on nucleosomal
查看所有相關論文

文章

Histone Modification and Chromatin Remodeling | Epigenetics

Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.

條款

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

相關內容

Protein & Nucleic Acid Interactions

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

Protein & Nucleic Acid Interactions

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務