BI0166

Activator Reagent 0.3M BMT in AcN

Biosynthesis Novabiochem^®

• 分類程式碼代碼: 41116006

屬性

• 品質等級: 200
• 產品線: Novabiochem^®
• 形狀: liquid
• 反應適用性: reaction type: Coupling Reactions; reagent type: catalyst
• 轉變溫度: flash point 2 °C (Acetonitrile)
• 運輸包裝: ambient
• 儲存溫度: 2-30°C

描述

一般說明

Activator Reagent 0.3M 5-benzylmercaptotetrazole (BMT) in acetonitrile (AcN) is also known as benzylthiotetrazole (BTT) or benzyl-1H-tetrazole). It is used as a phosphoramidite activator reagent for oligonucleotide synthesis.

應用

0.3M BMT in AcN can be used as a reagent in solid phase phosphorylation of the oligomers. The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the 5′hydroxyl group of the growing oligonucleotide chain. The high activation potential of this reagent may be due to its greater acidity (pKa=4.08), which makes it easier to protonate the trivalent phosphorus in the initial stage of activation and increase the speed of the coupling reaction.

特點和優勢

• efficient and rapid activation reagent that produces less
• phosphoramidite waste      
• higher solubility at lower temperatures      
• does not crystallize or clog lines       
• safer reagent to use

分析報告

Color (APHA): 10APHA max
Component: Acetonitrile Purity (By GC): 99.9% min
Form: Clear Liquid
Identity (IR-spectrum): Conforms
Molarity of 5-Benzylmercaptotetrazole: 0.28-0.32M
Water (H2O): 1-30ppm

法律資訊

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 象形圖: GHS02,GHS07
• 訊號詞: Danger
• 危險聲明: H225,H302 + H312 + H332,H319
• 防範說明: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• 危險分類: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
• 儲存類別代碼: 3 - Flammable liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: 35.6 °F - closed cup - (Acetonitrile)
• 閃點（°C）: 2 °C - closed cup - (Acetonitrile)