ABS82-AF488

Anti-VEGF Antibody, Alexa Fluor^™ 488 Conjugate

from rabbit, ALEXA FLUOR^™ 488

• 同義詞: Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.45
• 共軛: ALEXA FLUOR^™ 488
• application: ICC
• 無性繁殖: polyclonal
• 物種活性: rat, human
• 技術: immunocytochemistry: suitable

屬性

• 生物源: rabbit
• 品質等級: 100
• 共軛: ALEXA FLUOR^™ 488
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: rat, human
• 物種活性（以同源性預測）: nonhuman primates (based on 100% sequence homology)
• 技術: immunocytochemistry: suitable
• 同型: IgG
• NCBI登錄號: NP_001020537
• UniProt登錄號: P15692
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... VEGFA(7422)

描述

一般說明

VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic factor. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties, such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF also enhances permeabilization of blood vessels and plays a central role in the regulation of vasculogenesis. VEGF expression has been linked to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.

特異性

Other homologies: Bovine (85% sequence homology). Rat (70% sequence homology). Mouse (65% sequence homology).

This antibody recognizes VEGF at the N-terminus.

免疫原

Epitope: N-terminus.

KLH-conjugated linear peptide corresponding to human VEGF at the N-terminus.

應用

Anti-VEGF Antibody, Alexa Fluor^™ 488 Conjugate is an antibody against VEGF for use in Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VEGF in HeLa and A431 cells.
The unconjugated antibody (Cat. No. ABS82) is shown to be suitable also for Western blotting and immunohistochemistry (Paraffin) applications.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

品質

Evaluated by Immunocytochemistry in NIH/3T3 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected VEGF in NIH/3T3 cells.

標靶描述

~55 kDa observed.

外觀

Affinity purified

Purified rabbit polyclonal antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable