ABS1668M

Anti-CHMP3

serum, from rabbit

• 同義詞: Charged multivesicular body protein 3, Chromatin-modifying protein 3, Neuroendocrine differentiation factor, Vacuolar protein sorting-associated protein 24, hVps24
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 技術: immunocytochemistry: suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_057163
• UniProt登錄號: Q9Y3E7
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CHMP3(51652)

描述

一般說明

Charged multivesicular body protein 3 (UniProt: Q9Y3E7; also known as Chromatin-modifying protein 3, CHMP3, Neuroendocrine differentiation factor, Vacuolar protein sorting-associated protein 24, hVps24) is encoded by the CHMP3 (also known as CGI149, NEDF, VPS24, CGI-149) gene (Gene ID: 51652) in human. CHMP3 is a component of the endosomal sorting required for transport complex III (ESCRT-III), which is involved in multi-vesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. It is widely expressed and its expression is reported in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. The acidic C-terminus and the basic N-terminus of CHMP3 are thought to render the protein in a closed, soluble and inactive conformation through an auto-inhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. CHMP3 is shown to heteromerize with CHMP2A to form helical tubular structures that expose membrane-interacting sites on the outside. It can also interact with CHMP4A, however, the interaction requires the release of CHMP4A auto-inhibition.

特異性

This rabbit polyclonal antibody detects Charged multivesicular body protein 3 in human cells.

免疫原

A full-length human recombinant charged multivesicular body protein 3 (CHMP3).

Epitope: unknown

應用

Anti-CHMP3, Cat. No. ABS1668, is a rabbit polyclonal antibody that detects Charged multivesicular body protein 3 and has been tested for use in Immunocytochemistry and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected human recombinant charged multivesicular body protein 3 (CHMP3).

Western Blotting Analysis: A representative lot detected CHMP3 in Western Blotting applications (Morita, E., et. al. (2010). Proc Natl Acad Sci USA. 107(29):12889-94).

品質

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected CHMP3 in HeLa cells.

標靶描述

~32 kDa observed; 25.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Unpurified

Rabbit polyclonal antiserum with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1