ABE490

Anti-NSD2 Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Nuclear SET domain-containing protein 2, NSD2, Histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein, MMSET, Protein trithorax-5 and Wolf-Hirschhorn syndrome candidate 1 protein, WHSC1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 技術: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI登錄號: NP_001035889.1
• UniProt登錄號: O96028
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... WHSC1(7468)

描述

一般說明

Nuclear SET domain-containing protein 2 (NSD2) is also called Histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein (MMSET), Protein trithorax-5 and Wolf-Hirschhorn syndrome candidate 1 protein (WHSC1). NSD2 is a histone methyltransferase that acts on histone H3 ′Lys-27′ (H3K27me). Isoform RE-IIBP is thought to be a transcription regulator that uses HADC recruitment to binding DNA and suppress IL5 transcription. Chromosomal aberrations to WHSC1 are linked to multiple myeloma tumors.

特異性

This antibody is expected to cross-react with isoforms 1 (152 kDa), 3 (71 kDa), and 4 (66 kDa, REII-BP; IL-5 promoter REII-region-binding protein) based upon immunogen design.

免疫原

KLH-conjugated linear peptide corresponding to a region near the C-terminus of human NSD2.

應用

Detect NSD using this rabbit polyclonal antibody, Anti-NSD2 Antibody validated for use in western blotting, IHC & IP.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NSD2 in human cerebellum and thalamus tissues.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected NSD2 in human kidney tissues.

Immunoprecipitation Analysis: 5 µg of this antibody immunoprecipitated NSD2 from 500 µg of HCT-116 cell lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected NSD2 in 10 µg of HeLa cell lysate.

標靶描述

~150 kDa observed. Uniprot describes 7 different isoforms ranging from 30 kDa to 152 kDa

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable