推薦產品
生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
human
物種活性(以同源性預測)
mouse (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology)
包裝
antibody small pack of 25 μg
技術
immunocytochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... NFE2L2(4780)
一般說明
NF-E2相关因子2(核因子红系2相关因子2)是一种转录激活因子,可与目标基因启动子区域的抗氧化反应元件(ARE)结合。NF-E2相关因子2可介导基因对氧化应激的协同上调。NF-E2相关因子2也可能参与β-珠蛋白簇基因的转录激活。NF-E2相关因子2广泛表达,在成年肌肉、肾脏、肺、肝和胎儿肌肉中表达量最高。
特異性
该抗体可识别NF-E2相关因子2的C末端
免疫原
与人NF-E2相关因子2 C端相对应的KLH偶联肽。
表位:C末端
應用
抗NF-E2相关因子2抗体是一种高度特异性的兔多克隆抗体,靶向NF-E2相关因子2 & 已通过蛋白质印迹和&ICC测试。
研究子类别
转录因子
转录因子
研究类别
表观遗传学&核功能
表观遗传学&核功能
蛋白印迹分析: 1 µg/mL代表性批次产品在10 µg Jurkat细胞裂解液中检测到NF-E2相关因子2。
免疫细胞化学分析:一个代表性批次产品以1:200的稀释度,在未经处理和过氧化氢处理的细胞中检测到NF-E2相关因子2(注意:细胞质染色在氧化应激下增加。)
免疫细胞化学分析:一个代表性批次产品以1:200的稀释度,在未经处理和过氧化氢处理的细胞中检测到NF-E2相关因子2(注意:细胞质染色在氧化应激下增加。)
品質
通过蛋白质印迹对Ramos细胞裂解液进行了评估。
蛋白质印迹分析:1 µg/mL该抗体在10 µg Ramos细胞裂解液中检测到NF-E2相关因子2。
蛋白质印迹分析:1 µg/mL该抗体在10 µg Ramos细胞裂解液中检测到NF-E2相关因子2。
標靶描述
观测值~75 kDa。已知存在68 kDa(异构体1)和66 kDa(异构体2)的两个异构体。由于在Nrf2中发现了大量酸性残基,这导致其在SDS-PAGE中发生异常迁移,因此在〜75 kDa处观察到该蛋白质(Abu-Bakar, A., et al. (2007) Drug Metab Dispos.35(5):787-794.)。NF-E2相关因子2的泛素化形式可在〜110 kDa处观察到(Li, J., et al. (2005).Toxicol Sci. 83(2):313-328.)。
外觀
亲和纯化
纯化的兔单克隆抗体,溶于含有0.1 M Tris-甘氨酸(pH 7.4)、150 mM NaCl和0.05%叠氮化钠的缓冲液中。
儲存和穩定性
自发运之日起,在 2-8°C 条件下可稳定保存1年
分析報告
对照
Ramos细胞裂解液
Ramos细胞裂解液
其他說明
浓度:请参考批次特异性浓缩物的分析证书。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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