ABE1865

Anti-UTX (KDM6A)

from rabbit, purified by affinity chromatography

• 同義詞: Lysine-specific demethylase 6A, Histone demethylase UTX, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human, mouse
• 技術: ChIP: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI登錄號: NP_066963
• UniProt登錄號: O15550
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... KDM6A(7403)

描述

一般說明

Lysine-specific demethylase 6A (UniProt O15550; also known as Histone demethylase UTX, KDM6A, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein) is encoded by the KDM6A (also known as KABUK2, UTX) gene (Gene ID 7403) in human KDM6A associates with H3K4 methyltransferase MLL3/4 complex and specifically demethylates Lys-27 of histone H3 and plays a central role in histone code. It is shown to demethylate trimethylated and dimethylated, but not monomethylated H3 Lys-27. KDM6A mediates muscle regeneration through it demethylase activity, while it regulates mdsoderm differentiation of embryonic stem cells and mammary gland development independent of its demethylase activity. KDM6A also plays a potential role in regulating brown adipocyte thermogenic program. It is up-regulated during brown adipocyte differentiation and by cold exposure in both brown and white adipose tissue in mice, indicating its potential role in thermogenesis. Inactivation of KDM6A down-regulates brown fat specific gene expression. Mutations in KDM6A gene are a causative factor in Kabuki syndrome that is characterized by congenital mental retardation, postnatal dwarfism, large prominent earlobes, a cleft or high-arched palate, scoliosis, and other abnormalities.

Ref.:
Lederer, D et al. (2012). Am. J. Hum. Genet. 90, 119-124.
Zha, L et al. (2015). J. Biol. Chem. 290, 25151-25163.

特異性

This polyclonal antibody detected the expected UTX target band in wild-type, but not Utx-knockout murine ESC nuclear extract (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).

免疫原

His-tagged recombinant fragment within the central region of human UTX (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).

應用

Anti-UTX (KDM6A), Cat. No. ABE1865, is a highly specific rabbit polyclonal antibody that targets Lysine-specific demethylase 6A and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western blotting.

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected UTX (KDM6A) recruiment to the Myog, CKm, and Tnnc2 promoters following myogenic differentiation of primary murine myoblasts from wild-type or Utx(KI/KI) female mice harboring homozygous demethylase dead Utx mutant gene knock-in (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected a time-dependent UTX (KDM6A) enrichement at the Myogenin and CKm regulatory regions following myogenic differentiation of murine C2C12 myoblasts. shRNA-mediated Six4 knockdown, but not p38 MAPK inhibition, downregulated UTX (KDM6A) recruitment to the Myogenin and CKm loci (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).

Immunocytochemistry Analysis: A representative lot detected UTX (KDM6A) immunoreactivity by indirect immunofluorescence staining of 1% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized myofibers isolated from mouse extensor digitorum longus (EDL) muscles (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).

Immunofluorescence Analysis: A representative lot detected UTX (KDM6A) immunoreactivity by indirect immunofluorescence staining of 4% paraformaldehyde-fixed, 1% Triton X-100-permeabilized, transverse OCT cryosections sections of mouse tibialis anterior skeletal muscle (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).

Immunoprecipitation Analysis: A representative lot co-immunoprecipitated PTIP and RbBP5 with UTX using embryonic stem cell (ESC) nuclear extracts from wild-type or Utx(KI/KI) mice harboring homozygous demethylase dead Utx mutant gene knock-in. No UTX/PTIP/RbBP5 complex was precipitated from Utx-knockout ESCs (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).

Immunoprecipitation Analysis: A representative lot co-immunoprecipitated Six4 with UTX (KDM6A) using nuclear extract from murine C2C12 myoblasts 24 hrs post myogenic differentiation (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).

Western Blotting Analysis: A representative lot detected UTX4 in myoblast whole cell lysates and embryonic stem cell (ESC) nuclear extracts from wild-type or Utx(KI/KI) mice harboring homozygous demethylase dead Utx mutant gene knock-in, as well as in UTX and MLL4 immunoprecipitates. No UTX target band was detected in Utx-knockout ESCs (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).

Western Blotting Analysis: A representative lot detected UTX (KDM6A) in nuclear extracts from human K562 and murine C2C12 myoblasts (24 hrs post myogenic differentiation), as well as in Six4 immunoprecipitates. shRNA treatment prior to nuclear extract preparation diminished target band detection (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).

Research Category
Epigenetics & Nuclear Function

品質

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected UTX (KDM6A) in 10 µg of HeLa nuclear extract.

標靶描述

~154 kDa observed. 154.2 kDa (human; UniProt O15550) and 154.4/157.5 kDa (mouse isoform 1/2; UniProt O70546) calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1