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Merck
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重要文件

ABC1444

Sigma-Aldrich

Anti-Sorting Nexin-11 Antibody

from rabbit, purified by affinity chromatography

同義詞:

Sorting nexin-11, SNX11

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

mouse, human

物種活性(以同源性預測)

rat (based on 100% sequence homology)

技術

immunoprecipitation (IP): suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

mouse ... Snx11(74479)

一般說明

Sorting nexin-11 (UniProt Q91WL6) is encoded by the Snx11 gene (Gene ID 74479) in murine species. Sorting nexins (SNXs) are involved in diverse intracellular endosomal traffickings. Their phox homology (PX) domain binds certain phosphatidylinositols (PIs) and mediates their recruitment to PI-rich vesicles. Most SNXs contain additional recognizable domains and are referred to as SNX PX-BAR or SNX PX-other, while SNX3, SNX10, SNX11, SNX12, SNX22, and SNX24 contain only the PX domain and are referred to as SNX PX. SNX11 is found to contain a novel PXe domain (a.a. 16-132 of human and mouse SNX11) with two additional α-helices next to the conventional PX domain structure composed of three β-strands and three α-helices. F2RL1 (a.k.a. PAR2) is a protease-activated GPCR highly expressed throughout the central and the peripheral nervous system. F2RL1 is reported to translocate from the plasma membrane to nucleus upon stimulation via a microtubule-dependent shuttle machinery that requires sorting nexin 11.

特異性

This polyclonal antibody reacts with both human and murine sorting nexin-11. The immunogen sequence is also present in human spliced isoform 2 and rat sorting nexin-11.

免疫原

Epitope: Internal (C-terminal half).
Linear peptide corresponding to an internal sequence from the C-terminal half of mouse sorting nexin-11.

應用

Anti-Sorting Nexin-11 Antibody is an antibody against Sorting Nexin-11 for use in Western Blotting, Immunoprecipitation.
Research Category
Apoptosis & Cancer
Research Sub Category
Vesicular Trafficking
Western Blotting Analysis: 3.75 µg/mL from a representative lot detected sorting nexin-11 in 10 µg of HEK293T cell lysate.
Western Blotting Analysis: A representative lot detected reducing amount of sorting nexin-11 in lysates from HEK293 cells that had been transfected with SNX11 shRNA (Joyal, J.S., et al. (2014). Nat. Med. 20(10):1165-1173).
Western Blotting Analysis: A representative lot detected the presence of sorting nexin-11 in F2RL1-GFP fusion affinity pull-down only when using lysates of F2RL1-GFP-transfected HEK293 cells that had been stimulated with the F2RL1 agonist peptide (F2RL1-AP) SLIGKV, but not unstimulated F2RL1-GFP transfectants (Joyal, J.S., et al. (2014). Nat. Med. 20(10):1165-1173).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated importin-β1 (Impβ1) and F2RL1 with sorting nexin-11 from lysates of HEK293 cells that had been stimulated with the F2RL1 agonist peptide (F2RL1-AP) SLIGKV (Joyal, J.S., et al. (2014). Nat. Med. 20(10):1165-1173).

品質

Evaluated by Western Blotting in MCF-7 membrane extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected sorting nexin-11 in 10 µg of MCF-7 membrane extract.

標靶描述

~35 kDa observed. 30.43 kDa (human & mouse isoform 1) and 29.42 kDa (human isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

外觀

Affinity purified
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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