ABC137

Anti-ATPIF1 Antibody

from rabbit, purified by affinity chromatography

• 同義詞: ATPase inhibitor, mitochondrial, Inhibitor of F(1)F(o)-ATPase, IF(1), IF1, ATPIF1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human, mouse
• 技術: immunocytochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_057395
• UniProt登錄號: Q9UII2
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ATPIF1(93974)

描述

一般說明

ATPIF1 is an interesting protein that acts on ATP synthase and prevents it from catabolizing ATP when the mitochondrial membrane potential falls below a threshold, which ATP synthase starts to do by hydrolyzing ATP to pump protons out of the mitochondrial matrix to restore ion balance. ATPIF1 also indirectly regulates heme synthesis by modulating the mitochondrial pH and redox potential. ATPIF1 is expressed in the mitochondria and its active is determined by conformation. Homodimers are active, homotetramers are inactive and their formation is governed by pH. ATPIF1, because of its effects on ATP and heme, is important in cellular development, heme biosynthesis, energy metabolism and angiogenesis and erythrocytes differentiation.

免疫原

Epitope: N-terminal inhibitory region & Antiparallel alpha-helical coiled region

GST-tagged recombinant protein corresponding to the N-terminal inhibitory region & Antiparallel alpha-helical coiled eegion of human ATPIF1.

應用

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-ATPIF1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of ATPIF1.

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected ATPIF1 in 10 µg of HEK293 cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected ATPIF1 in HeLa, A431, and NIH/3T3 cells.

品質

Evaluated by Western Blotting in T47D cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected ATPIF1 in 10 µg of T47D cell lysate.

標靶描述

~12 kDa observed. Uncharacterized band(s) may appear in some lysates.

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable