跳轉至內容
Merck
全部照片(1)

重要文件

AB5454

Sigma-Aldrich

Anti-TUC-4 Protein Antibody

serum, Chemicon®

同義詞:

ULIP-1 Protein, CRMP-4, DRP-3, Dihydropyrimidinase-like 3

登入查看組織和合約定價


About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

serum

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

feline, monkey, human, mouse, rat

製造商/商標名

Chemicon®

技術

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

human ... DPYSL3(1809)

一般說明

TUC (TOAD [Turned On After Division]/ Ulip/CRMP) is a family of proteins that have emerged as strong candidates for a role in neuronal growth cone signaling. The TUC-4 family members reach their highest expression levels in early post-mitotic neurons during their peak periods of axonal growth. TUC-4 is not expressed in neural progenitors or adult neurons (with minor exception). Data suggests that TUC-4 plays a role in axonal outgrowth, pathfinding and possibly in the process that permits growing axons to choose proper routes and targets (Quinn, 1999). The TUC-4 protein is expressed primarily within the cytoplasm of postmitotic neurons as they begin their migration out of the ventricular zone into the developing cortical plate (Minturn, 1995a). TUC-4 is expressed in neuronal cell bodies, neurites, and along the most distal regions of neuronal growth cones, including the lamellipodia and filopodia (Minturn, 1995b). Expression of TUC-4 is observed by day E12 in rats coincident with the expression of the early neuronal marker class III beta-tubulin (Quinn, 1999). TUC-4 expression is rarely observed in neuronal cells expressing the mitotic marker PCNA (Minturn, 1995a). The expression of TUC-4 has been shown to be upregulated in response to NGF in rats (Minturn, 1995b) and in response to retinoic acid in a human neuroblastoma cell line (SMS-KCNR; Gaetano, 1997).

特異性

Specifically recognizes the TUC-4 (Ulip-1/CRMP-4/DRP-3) splice variants, TUC-4a (64 kDa) and the N-terminally extended TUC-4b (75 kDa) (Quinn, 2003). AB5454 has been reported to label early postmitotic neurons in prenatal rat brain. Labeling is observed primarily within the cytoplasm along the most distal regions of neuronal growth cones including lamellipodia and filopodia. The antibody is not reactive with the proteins TUC-1 (Ulip-3/CRMP-1), TUC-2 (Ulip-2/CRMP-2) or TUC-3 (Ulip-4/CRMP-3). It does not label neural progenitors or radial glia.

免疫原

Synthetic peptide corresponding to amino acids 499 to 511 of TUC-4a and amino acids 612-624 of TUC-4b (YDGPVFDLTTTPK)

應用

Anti-TUC-4 Protein Antibody detects level of TUC-4 Protein & has been published & validated for use in IC, IH, IP & WB.
Immunohistochemistry: 1:1,000-1:5,000 using PFA (DAB detection).
Immunocytochemistry: 1:1,000-1:5,000 (DAB detection).
Western blot: 1:2,500-1:25,000 using alkaline phosphatase.
Immunoprecipitation

Optimal working dilutions must be determined by end user.

標靶描述

64 & 74 kDa

外觀

Rabbit Serum. Liquid containing 0.2% sodium azide.

分析報告

Control
POSITIVE CONTROL: Fetal brain/spinal cord tissue. Human neuroblastoma cell line SMS-KCNR or rat PC12 cells.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

未找到適合的產品?  

試用我們的產品選擇工具.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Nandrolone-induced hippocampal phosphorylation of NMDA receptor subunits and ERKs.
Uwe L W Rossbach,Pia Steensland,Fred Nyberg,Pierre Le Greves
Biochemical and biophysical research communications null
Christopher C Quinn et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(7), 2815-2823 (2003-04-10)
The TUC (TOAD-64/Ulip/CRMP) proteins are homologs of UNC-33, a protein that is required for axon extension and guidance in Caenorhabditis elegans. The TUC proteins are expressed in newly born neurons in the developing nervous system and have been implicated in
Cystatin C modulates neurodegeneration and neurogenesis following status epilepticus in mouse.
Terhi J Pirttila, Katarzyna Lukasiuk, Katarina H?kansson, Anders Grubb, Magnus Abrahamson et al.
Neurobiology of Disease null
Maturational sequence of newly generated neurons in the dentate gyrus of the young adult rhesus monkey.
Laura B Ngwenya, Alan Peters, Douglas L Rosene
The Journal of Comparative Neurology null
Proteomic analysis of gamma-butyrolactone-treated mouse thalamus reveals dysregulated proteins upon absence seizure.
Myung-Jeom Ryu, Daesoo Kim, Un-Beom Kang, Joon Kim, Hee-Sup Shin, Cheolju Lee, Myeong-Hee Yu
Journal of Neurochemistry null

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務