AB3843

Anti-Ezrin/Radixin/Moesin Antibody

Chemicon^®, from rabbit

• 同義詞: Anti-EBP50, Anti-NHERF, Anti-NHERF-1, Anti-NHERF1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: mouse, monkey, rat, human
• 製造商／商標名: Chemicon^®
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI登錄號: NM_004252.2
• UniProt登錄號: O14745
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SLC9A3R1(9368)

描述

特異性

Recognizes Ezrin/Radixin/Moesin. These proteins function as linkers between the plasma membrane and the actin cytoskeleton, and are involved in cell adhesion, membrane ruffling, and microvilli formation. This antibody does not cross-react with other related proteins such as merlin or band 4.1.

免疫原

KLH-conjugated, synthetic peptide corresponding to residues surrounding Thr567 of human ezrin.

應用

Detect Ezrin/Radixin/Moesin using this Anti-Ezrin/Radixin/Moesin Antibody validated for use in WB, IC, IH(P).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western blotting: 1:1,000. The 75 kDa band is Moesin and the 80kDa band is Ezrin and Radixin. (For best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight)

Immunohistochemistry (Paraffin): 1:50. Positive control tissue is breast carcinoma. Cellular localization is membrane and cytoplasm

Immunocytochemistry (IF): 1:100

Optimal working dilutions must be determined by the end user.

外觀

Protein A and peptide affinity purified immunoglobulin. Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol.

儲存和穩定性

Maintain at -20ºC for 12 months, do not aliquot.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2