推薦產品
生物源
rabbit
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
human, rat, mouse
製造商／商標名
Chemicon®
技術
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... HSP90AA1(3320)
mouse ... Hsp90Aa1(15519)
rat ... Hsp90Aa1(299331)
特異性
Reacts with a protein of 84 kD identified as heat shock protein 90beta (HSP90beta). No reactivity with HSP90alpha/HSP86.
CELLULAR LOCALIZATION: Nuclear and Cytoplasmic.
CELLULAR LOCALIZATION: Nuclear and Cytoplasmic.
免疫原
Synthetic peptide corresponding to amino acids 2-13 from the N-terminus of mouse heat shock protein 90b.
應用
Immunoblotting: 5 μg/mL
Immunohistochemistry (frozen and formalin/paraffin): 5-10 μg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunocytochemistry
Immunoprecpitation: 10 μg/mg of protein lysate.
Optimal working dilutions must be determined by end user.
Immunohistochemistry (frozen and formalin/paraffin): 5-10 μg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunocytochemistry
Immunoprecpitation: 10 μg/mg of protein lysate.
Optimal working dilutions must be determined by end user.
Research Category
Protein Trafficking
Protein Trafficking
Research Sub Category
Chaperones
Chaperones
This Anti-Heat Shock Protein 90β Antibody is validated for use in IP, WB, IC, IH(P) for the detection of Heat Shock Protein 90β.
外觀
Format: Purified
Purified immunoglobulin. Liquid in 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide.
儲存和穩定性
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
分析報告
Control
POSITIVE CONTROL: MAD109 cells or breast carcinoma.
POSITIVE CONTROL: MAD109 cells or breast carcinoma.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Malignant transformation in a defined genetic background: proteome changes displayed by 2D-PAGE.
Putz, SM; Vogiatzi, F; Stiewe, T; Sickmann, A
Molecular Cancer null
Detection of anti-heat shock protein 90 beta (Hsp90beta) antibodies in cerebrospinal fluid.
C Cid, M Garcia-Villanueva, M Salinas, A Alcazar
Journal of Immunological Methods null
Signalling profile and antitumour activity of the novel Hsp90 inhibitor NVP-AUY922 in multiple myeloma.
Stuhmer, T; Zollinger, A; Siegmund, D; Chatterjee, M; Grella, E; Knop, S; Kortum et al.
Leukemia null
Ying-Li Wu et al.
Journal of cell science, 119(Pt 13), 2797-2806 (2006-06-15)
The involvement of telomerase in cellular immortalization and senescence has often been assessed by means of telomerase expression at the RNA level and quantification of telomerase activity by the telomeric repeat amplification protocol assay. However, these methods either neglected the
Heat shock protein 90 overexpression independently predicts inferior disease-free survival with differential expression of the alpha and beta isoforms in gastrointestinal stromal tumors.
Li, CF; Huang, WW; Wu, JM; Yu, SC; Hu, TH; Uen, YH; Tian, YF; Lin, CN; Lu, D; Fang, FM; Huang, HY
Clinical cancer research : an official journal of the American Association for Cancer Research null
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