推薦產品
生物源
chicken
品質等級
抗體表格
affinity purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
rat
製造商／商標名
Chemicon®
技術
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
rat ... Aqp9(65054)
一般說明
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane.
A new member of AQP family, Aquaporin-9 (AQP9), has been cloned from human leukocytes by homology cloning (Ishibashi et al. 1997). It is mostly homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295 AA) is primarily expressed in peripheral leukocytes, and lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it did not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.
A new member of AQP family, Aquaporin-9 (AQP9), has been cloned from human leukocytes by homology cloning (Ishibashi et al. 1997). It is mostly homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295 AA) is primarily expressed in peripheral leukocytes, and lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it did not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.
特異性
Aquaporin 9. The immunogen peptide is unique to Aquaporin-9 without significant homology to any other Aquaporins.
Rat. The immunogen peptide has 94% conservation with mouse and 72% conservation with the human Aquaporin-9. Species reactivity has not been confirmed.
Rat. The immunogen peptide has 94% conservation with mouse and 72% conservation with the human Aquaporin-9. Species reactivity has not been confirmed.
免疫原
An 18 AA synthetic peptide within the C-terminal domain of rat Aquaporin-9 (Ishibashi et al. 1997) was selected for antibody production. This domain is predicted to be cytoplasmic.
應用
Detect Aquaporin 9 using this Anti-Aquaporin 9 Antibody validated for use in ELISA, IC, IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Western blot: 1-10 μg/mL using ECL
ELISA: 1:100,000 using 50-100 ng of control peptide per well.
Immunohisto/cytochemistry: We recommend using the affinity purified antibody at 2-10 μg/mL in paraformaldehyde fixed sections of tissues. Adherent cells can be fixed with 50% methanol-50% acetone or 1% paraformaldehyde.
Optimal working dilutions must be determined by end user.
ELISA: 1:100,000 using 50-100 ng of control peptide per well.
Immunohisto/cytochemistry: We recommend using the affinity purified antibody at 2-10 μg/mL in paraformaldehyde fixed sections of tissues. Adherent cells can be fixed with 50% methanol-50% acetone or 1% paraformaldehyde.
Optimal working dilutions must be determined by end user.
外觀
Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
儲存和穩定性
Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Agmatine Attenuates Brain Edema and Apoptotic Cell Death after Traumatic Brain Injury.
Kim, JY; Lee, YW; Kim, JH; Lee, WT; Park, KA; Lee, JE
Journal of Korean Medical Science null
Elizabeth Kimball et al.
PloS one, 16(2), e0244123-e0244123 (2021-02-03)
To study aquaporin channel expression in astrocytes of the mouse optic nerve (ON) and the response to IOP elevation in mice lacking aquaporin 4 (AQP4 null). C57BL/6 (B6) and AQP4 null mice were exposed to bead-induced IOP elevation for 3
Elizabeth C Kimball et al.
PloS one, 17(6), e0268541-e0268541 (2022-06-17)
Aquaporin 4 is absent from astrocytes in the rodent optic nerve head, despite high expression in the retina and myelinated optic nerve. The purpose of this study was to quantify regional aquaporin channel expression in astrocytes of the porcine and
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