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重要文件

AB1597P

Sigma-Aldrich

Anti-Vesicular Monoamine Transporter 1 Antibody

Chemicon®, from rabbit

同義詞:

VMAT1

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

mouse, rat

製造商/商標名

Chemicon®

技術

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

特異性

Vesicular Monoamine Transporter 1 (VMAT1). An antibody made to the C-terminal VMAT1 peptide detected a major band at ~55 kDa in postnuclear supernatants of CHO transfected with VMAT1 and not in wild type cells (Liu et al. 1994). Some additional bands, both higher and lower molecular weight, were also detected and remain unaffected by the inclusion of protease inhibitors. VMAT1 has been localized in adrenal chromaffin cells, endocrine and paracrine cells associated with the intestine, stomach and sympathetic nervous system (Liu et al. 1994; Weihe et al. 1994). VMAT1 has also been found in endosomes of transfected CHO cells and in LDCVs of rat PC12 cells (Liu et al. 1994). This antibody is not expected to cross-react with human VMAT1 (Erickson et al. 1996).

免疫原

A 12 amino acid C-terminal peptide sequence from rat VMAT1 (Liu et al. 1992). This peptide is predicted to be cytoplasmic.

應用

Anti-Vesicular Monoamine Transporter 1 Antibody detects level of Vesicular Monoamine Transporter 1 & has been published & validated for use in ELISA, IH & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot: 1-10 μg/mL (Chemiluminescence technique)

Immunohistochemistry: 1-20 μg/mL on paraformaldehyde fixed sections.

ELISA: 0.5-1.0 μg/mL (1 μg/mL VMAT1 peptide (Catalog Number AG250)/well)

Optimal working dilutions must be determined by end user.

外觀

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA.

儲存和穩定性

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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存取文件庫

Vesicular monoamine transporter 1 mediates dopamine secretion in rat proximal tubular cells.
Maurel, A; Spreux-Varoquaux, O; Amenta, F; Tayebati, SK; Tomassoni, D; Seguelas et al.
American Journal of Physiology: Renal Physiology null
Alex Ismail et al.
FEBS letters, 591(1), 137-144 (2016-12-08)
Rat perivascular adipose tissue (PVAT) stores, takes up, and releases norepinephrine (NE; Ayala-Lopez et al. (2014) Pharmacol Res Perspect 2, e00041). We hypothesized that 3T3-L1 adipocytes would exhibit similar behaviors and, thus, could serve as a model for PVAT adipocytes.

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