8.52309

Fmoc-ACA-OH

Novabiochem^®

• 同義詞: Fmoc-ACA-OH, 7-N-Fmoc-aminocoumarin-4-acetic acid
• 經驗公式（希爾表示法）: C₂₆H₁₉NO₆
• CAS號碼: 378247-75-7
• 分子量：: 441.43
• 分類程式碼代碼: 12352209
• NACRES: NA.22

屬性

• 品質等級: 200
• 產品線: Novabiochem^®
• 化驗: ≥95.0% (HPLC); ≥98% (TLC)
• 形狀: powder
• 反應適用性: reaction type: Fmoc solid-phase peptide synthesis
• 製造商／商標名: Novabiochem^®
• 應用: peptide synthesis
• 官能基: aromatic amine
• 儲存溫度: 2-8°C
• InChI: 1S/C26H19NO6/c28-24(29)11-15-12-25(30)33-23-13-16(9-10-17(15)23)27-26(31)32-14-22-20-7-3-1-5-18(20)19-6-2-4-8-21(19)22/h1-10,12-13,22H,11,14H2,(H,27,31)(H,28,29)
• InChI 密鑰: RCUKLQDGAASIAX-UHFFFAOYSA-N

描述

一般說明

Fmoc-ACA-OH is a useful tool for the SPPS of AMC fluorogenic protease substrates by Fmoc SPPS. Fmoc-ACA-OH ^[1] must be first loaded onto a Wang-type resin. Any unreacted linker hydroxyls must be capped using acetic anhydride. Following Fmoc removal, the first amino acid should be coupled with HATU/collidine ^[2]. Insertion of ACA into the middle of a peptide provides fluorogenic substrates for endopeptidases ^[3]. Treatment of the ACC peptide obtained after TFA cleavage with an aqueous base causes facile decarboxylation and formation of the AMC peptide.

Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS
Chromogenic Labeling of Synthetic Peptides

Literature references

^[1] J. L. Harris, et al. (2000) Proc. Natl. Acad. Sci. USA, 97, 7754.
^[2] D. J. Maly, et al. (2002) J. Org. Chem., 67, 910.
^[3] C. M. Salisbury, et al. (2002) J. Am. Chem. Soc., 124, 14868.

分析報告

Color (visual): white to slight yellow to light red
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC(CMA1)): ≥ 98 %
Assay (HPLC, area%): ≥ 95.0 % (a/a)
Solubility (1 mmole in 2 ml DMF): clearly soluble

To see the solvent systems used for TLC of Novabiochem^® products please click here.

法律資訊

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable