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pETcoco-1 DNA - Novagen

Novagen′s pETcoco-1 expression vector is designed to allow "on command" amplification of vector copy number from single copy (1-2 plasmid copies per cell) to medium copy (20-50 per cell).

• 分類程式碼代碼: 41106602
• NACRES: NA.51

屬性

• 籤條: Herpes Simplex Virus (HSV) tagged; His tagged; S-Tag tagged
• 品質等級: 100
• 形狀: liquid
• 製造商／商標名: Novagen^®
• 儲存條件: OK to freeze
• 運輸包裝: wet ice
• 儲存溫度: −70°C

描述

一般說明

The pETcoco-1 expression vector is designed to allow "on command" amplification of vector copy number from single copy (1–2 plasmid copies per cell) to medium copy (20–50 per cell). The vector is maintained in the single copy state by utiliziing the oriS and repE elements of the F episome together with the parABC partition determinants. The medium copy state is obtained by employing the RK-2 derived trfA replicator acting at oriV. The switch from single copy to medium copy status is achieved by inducing the expression of the trfA gene with arabinose. The dual replicon nature of these vectors promotes enhanced stability of recombinant plasmids and extremely low basal expression levels in the single copy state, while allowing robust target gene expression typical of the pET vectors. Unique restriction sites are shown on the circle map. pETcoco-1 contains the chloramphenicol resistance gene. The sequence is numbered relative to the start of T7 transcription at +1.

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

成分

•10 µgpETcoco-1 DNA

•0.2 mlInduction Control Glycerol Stock

警告

Toxicity: Standard Handling (A)

其他說明

Vector Family: pETcoco

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable