6502

Trypsin, Bovine Pancreas

• 同義詞: Trypsin, Bovine Pancreas
• CAS號碼: 9002-07-7
• 酶委員會編號: 3.4.21.4 (BRENDA, IUBMB)
• MDL號碼: MFCD00040513
• 分類程式碼代碼: 12352202
• NACRES: NA.25

屬性

• 描述: RTECS - YN5075000; Merck USA index - 14, 9795
• 品質等級: 100
• 形狀: solid
• 比活性: ≥2,500 USP units/mg dry wt
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; desiccated (hygroscopic)
• 溶解度: HCl: 1 mM; neutral buffer: soluble; water: soluble
• 異物活動: Chymotrypsin ≤50 NF units/mg dry wt.
• 運輸包裝: ambient
• 儲存溫度: −20°C

描述

一般說明

Native trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Has an optimal pH of 8.5-8.8.

Note: 1 NF unit = 1 USP unit = 3 SU at 30°C, pH 7.6; 1 TAME unit = 19.2 USP units = 57.5 BAEE units; 1 MU = 1,000,000 units.

警告

Toxicity: Irritant (B)

單位定義

One USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.

外觀

Salt-free

重構

Following reconstitution in HCl, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Following reconstitution in H₂O or buffer, use immediately or aliquot and freeze (-70°C) to minimize autolysis. HCl stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. H₂O or buffer stock solutions are stable for up to 1 month at -70°C.

其他說明

Bergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3^rd ed. Vol. 3, 17.
Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem.239, 1799.
Hummel, B.C.W. 1955. Can. J. Biochem. Physiol.37, 1393.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 象形圖: GHS08,GHS07
• 訊號詞: Danger
• 危險聲明: H315,H319,H334,H335
• 防範說明: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• 危險分類: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 標靶器官: Respiratory system
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1