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Merck
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48-660MAG

Millipore

MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay

同義詞:

Cell Signaling Kit, MAPK/SAPK 10-Plex Kit

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.47

物種活性

human

品質等級

200

製造商／商標名

Milliplex®

技術

multiplexing: suitable

assay range

inter-assay cv: 4-13%
intra-assay cv: 3-10%

檢測方法

fluorometric (Luminex xMAP)

儲存溫度

2-8°C

一般說明

Cells respond to their environment in various ways through intracellular signaling. Signaling through Receptor Tyrosine kinases (RTKs) often promotes increased metabolism and cell growth. Activation of ERK/MAP kinase is one of the key serine/threonine kinases activated via RTK signaling, promoting increased activity of p70 S6 kinase, MSK1, STATs, CREB, and many other signaling intermediates. Other signaling pathways induced via stress (ex. heat shock/arsenite treatment) or death receptors (ex. TNFα) promote p38, JNK, and p53 signaling pathways.

特異性

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

應用

  • An overnight (4°C) incubation is recommended for best results.
  • This assay requires 25 μL diluted cell lysate.
  • This kit must be run using Assay Buffer 2 (provided).
  • 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.
Research Category
Signaling
Used to detect/quantify:ATF2 (Thr71)|Erk (Thr185/Tyr187)|HSP27 (Ser78)|JNK (Thr183/Tyr185)|c-Jun (Ser73)|MEK1 (Ser222)|MSK1 (Ser212)|p38 (Thr180/Tyr182)|p53 (Ser15)|STAT1 (Tyr701)

法律資訊

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

分析證明 (COA)

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