407617

Interleukin-1β, Mouse, Recombinant, E. coli

• 分類程式碼代碼: 12352202
• NACRES: NA.41

屬性

• 描述: Merck USA index - 14, 4993
• 品質等級: 100
• 化驗: ≥97% (SDS-PAGE)
• 形狀: lyophilized
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 雜質: ≤1.0 EU/μg Endotoxin (EU/μg IL-1β)
• 運輸包裝: ambient
• 儲存溫度: −70°C

描述

一般說明

Recombinant, mouse interleukin-1β expressed in E. coli. Suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing. Interleukin-1 is a protein produced by stimulated macrophages and many other cell types. The following biological activities are attributed to IL-1: activation of T cells resulting in the production of IL-2 and expression of IL-2 receptors; initiation of Acute Phase Response by induction of hepatic protein synthesis; increased proteolysis of muscle proteins, amino acid mobilization, and caloric and oxygen utilization; induction of bone resorption; stimulation of fibroblast proliferation; induction of slow-wave sleep; fever production; induction of prostaglandin and leukotriene production; stimulation of thymocyte proliferation; induction of B cell proliferation and maturation and increased immunoglobulin synthesis; induction of collagenase and other proteinases in synovial fibroblasts and articular chrondrocytes, suggesting a role in pathological states such as arthritis; activation of neutrophils. 1 µg is equivalent to 1.1 x 10⁶ WHO units.

生化／生理作用

ED₅₀= 5-10 pg/ml as measured in a cell proliferation assay

警告

Toxicity: Standard Handling (A)

外觀

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-1β.

重構

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

其他說明

Chun, S.Y., et al. 1995. Endocrinology136, 3120.
Weidmann, B., et al. 1994. Nature370, 434.
Dinarello, C.A., and Wolff, S.M. 1993. N. Engl. J. Med.328, 106.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable