382061

Bisbenzimide H 33258 Fluorochrome, Trihydrochloride

Membrane-permeable, adenine-thymine-specific fluorescent stain.

• 同義詞: Bisbenzimide H 33258 Fluorochrome, Trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)-benzimidazole, 3HCl, HOE 33258
• 經驗公式（希爾表示法）: C₂₅H₂₄N₆O · 3HCl
• CAS號碼: 23491-45-4
• 分子量：: 533.88
• MDL號碼: MFCD00012679
• 分類程式碼代碼: 12352111
• NACRES: NA.21

屬性

• 品質等級: 100
• 描述: RTECS - SM1140500
• 化驗: ≥98% (HPLC)
• 形狀: solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; protect from light
• 顏色: yellow-green
• 螢光: λ_ex 346 nm; λ_em 460 nm
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• SMILES 字串: [Cl-].[Cl-].[Cl-].N1(CCN(CC1)c2cc3[nH]c(nc3cc2)c4cc5c(cc4)NC(=C6C=CC(=O)C=C6)N5)C.[H+].[H+].[H+]
• InChI: 1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,26,28H,10-13H2,1H3,(H,27,29);3*1H
• InChI 密鑰: OKQAASMPIOSOEX-UHFFFAOYSA-N

描述

一般說明

Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca²⁺ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.

Membrane-permeant, adenine-thymidine specific fluorescent stain (excitation maximum: 346 nm; emission maximum: 460 nm). Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca²⁺ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.

生化／生理作用

Cell permeable: yes

Primary Target
Adenine-thymine-specific fluorescent stain

Product does not compete with ATP.

Reversible: no

警告

Toxicity: Carcinogenic / Teratogenic (D)

準備報告

Precipitates in phosphate buffered solutions

重構

Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 weeks at 4°C.

其他說明

Werstuck, G., and Green, M.R. 1998. Science 282, 296.
Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.
Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.
Tarshis, M., et al. 1994. J. Immunol. Methods168, 245.
Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.
Oberhammer, F., et al. 1993. EMBO J. 12, 3679.
Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods162, 41.
Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA85, 8939.
Cesarone, C.F., et al. 1979. Anal. Biochem.100, 188.
Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem.24, 24.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable