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重要文件

371754

Sigma-Aldrich

Anti-Gqα- and G₁₁α-Subunits, Internal (283-300) Rabbit pAb

liquid, Calbiochem®

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

liquid

不包含

preservative

物種活性

human, mouse, rat

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

同型

IgG

運輸包裝

wet ice

儲存溫度

−70°C

目標翻譯後修改

unmodified

基因資訊

human ... GNAI1(2770)
mouse ... Gnai1(14677)
rat ... Gnai1(25686)

一般說明

Anti-Gqα- and G₁₁α-Subunits, Internal (283-300), rabbit polyclonal, recognizes the ~42 kDa Gqα- and the ~43 kDa G₁₁α-subunits. Validated for use in WB and neutralization studies.
Protein A purified rabbit polyclonal antibody. Recognizes the ~42 kDa Gqα- and the ~43 kDa G11α-subunit proteins.
Recognizes the ~42 kDa Gqα- and the ~43 kDa G11α-subunits. Does not cross-react with other G-proteins of the non-pertussis toxin-sensitive family.

免疫原

a synthetic peptide (IMYSHLVDYFPEYDGPQR) corresponding to an internal amino acid sequence shared by human, mouse, and rat Gqα- and G₁₁α-subunits

應用

Immunoblotting (1:1000)

Neutralization Studies (see comments)

警告

Toxicity: Standard Handling (A)

其他說明

The antibody recognizes both Gqα and G11α since they both share the internal sequence represented by the immunizing peptide. The antibody may also be useful to block receptor G protein coupling. Does not cross-react with other G proteins of the non-pertussis toxin sensitive family. Variables associated with assay conditions will dictate the proper working dilution.
Wange, R.L., et al. 1991. J. Biol. Chem. 266, 11409.
Pang, I-H., and Sternweis, P.C. 1990. J. Biol. Chem. 265, 18707.
Strathmann, M., et al. 1989. Proc. Natl. Acad. Sci. USA86, 7407.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1


分析證明 (COA)

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Wen Hu et al.
The Journal of biological chemistry, 287(17), 13911-13918 (2012-03-06)
Appropriate termination of the phototransduction cascade is critical for photoreceptors to achieve high temporal resolution and to prevent excessive Ca(2+)-induced cell toxicity. Using a genetic screen to identify defective photoresponse mutants in Drosophila, we isolated and identified a novel Gα(q)
Apurva A Javkhedkar et al.
Physiological reports, 3(11) (2015-11-26)
Previously we have shown that in spontaneously hypertensive rats (SHR) renal angiotensin (Ang) II receptor (AT1R) upregulation leads to overstimulation of Na/K-ATPase by Ang II. There are reports that antioxidants can reduce oxidative stress and blood pressure (BP) in SHR
Timo Littmann et al.
Analytical biochemistry, 573, 8-16 (2019-03-12)
Functional selectivity of agonists has gained increasing interest in G protein-coupled receptor (GPCR) research, e.g. due to expectations of drugs with reduced adverse effects. Different agonist-dependent GPCR conformations are conceived to selectively activate a balanced or imbalanced intracellular signalling response
Arnaud Ruiz et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 25(50), 11710-11718 (2005-12-16)
Heteromeric kainate receptors (KARs) containing both glutamate receptor 6 (GluR6) and KA2 subunits are involved in KAR-mediated EPSCs at mossy fiber synapses in CA3 pyramidal cells. We report that endogenous glutamate, by activating KARs, reversibly inhibits the slow Ca2+-activated K+

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