361527

PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3)

liquid, clone 2D3, Calbiochem^®

• 分類程式碼代碼: 12352203

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2D3, monoclonal
• 形狀: liquid
• 包含: ≤0.1% sodium azide as preservative (antibody only)
• 物種活性: mouse, human
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 同型: IgG1
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 目標翻譯後修改: phosphorylation (pSer9)
• 基因資訊: human ... GSK3B(2932) ; mouse ... Gsk3B(56637)

描述

一般說明

Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser⁹. Supplied with a control lysate derived from 2 x 10⁶ EGF-treated HepG2 cells.

Recognizes the ~46 kDa GSK-3β phosphorylated at Ser⁹. Supplied with a control lysate derived from 2 x 10⁶ EGF-treated HepG2 cells.

This PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer⁹).

免疫原

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β

應用

ELISA (0.05 µg/ml)

Immunoblotting (0.5 µg/ml)

警告

Toxicity: Standard Handling (A)

外觀

100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.

重構

Reconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.

分析報告

Positive Control
Pervanadate-treated HepG2 (included)

其他說明

For immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable